Ramos-Blue™ Cells

Ramos-Blue™ cells are B lymphocyte cell lines that stably expresses an NF-κB/AP-1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene.

Ramos-Blue™ cells are responsive to NF-κB inducers, such as TNF-α and some TLR agonists (poly(I:C), R848, ODN 2006). When stimulated, they produce SEAP in the supernatant. The levels of SEAPcan be readily monitored using QUANTI-Blue™, a medium that turns purple/blue in the presence of SEAP.

Figures for this product

NF-kB/AP-1 activation in Ramos-Blue™ cells induced by various activatorsMyD88 dependent and independent responses in Ramos-Blue™ and Ramos-Blue™-defMyD


Antibiotic resistance: Zeocin™

Growth medium: IMDM, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

Shipped on dry ice


1 vial containing 3-7 x 106 cells

100 μl Zeocin™ (100 mg/ml)

1 ml Normocin™ (50 mg/ml)

1 pouch of QUANTI-Blue™ (100 ml)


Recent articles using InvivoGen Ramos-Blue™ Cells


Ramos-Blue™ cells

Description NF-κB/AP-1 reporter B lymphocytes
Cat. Coderms-sp
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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