Poly(A:U)

Polyadenylic–polyuridylic acid

Polyadenylic–polyuridylic acid (poly(A:U)) is a synthetic double stranded RNA molecule that signals only through TLR3. Recognition of poly(A:U) by TLR3 induces the activation of dendritic cells and T lymphocytes.
When combined with an antigen in mice, poly(A:U) has been shown to promote antigen-specific Th1-immune responses and boost antibody production [1].
The potent adjuvant activity of poly(A:U) has been exploited in the treatment of breast cancers that express TLR3 [2].

Specifications

Specificity: TLR3 agonist

Working Concentration: 300 ng - 100 μg/ml

CAS number: 24936-38-7

Endotoxin level:  < 5 EU/mg

Solubility: 1 mg/ml in physiological water (NaCl 0.9%) 

Contents

• 10 mg sterile lyophilized poly(A:U)
• 10 ml sterile physiological water

Poly(A:U) is shipped at room temperature and should be stored at 4°C. Upon resuspension, Poly(A:U) should be aliquoted and stored at -20°C for long term storage. Store at 4°C for short term storage. Lyophilized product is stable 1 year at 4°C when properly stored. Resuspended product is stable 1 month at 4°C and 1 year at -20°C. Avoid repeated freeze-thaw cycles.

Ref.

1. Wang L. et al., 2002. Noncoding RNA danger motifs bridge innate and adaptive immunity and are potent adjuvants for vaccination. J Clin Invest 110:1175–84.
2. Conforti R. et al., 2010. Opposing effects of toll-like receptor (TLR3) signaling in tumors can be therapeutically uncoupled to optimize the anticancer efficacy of TLR3 ligands. Cancer Res. 70(2):490-500.

Citations

Recent articles using InvivoGen Poly(A:U)

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Poly(A:U)

Cat. Codetlrl-pau
Unit Size10 mg
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