HEK-Blue™ TLR3

HEK-Blue™ TLR3 cells are designed for studying the stimulation of TLR3 by monitoring the activation of NF-κB/AP1.

TLR3 recognizes double-stranded RNA (dsRNA), a molecular pattern associated with viral infection. Stimulation with poly(I:C), a synthetic analog of dsRNA, was shown to induce hyporesponsiveness in TLR3-deficient mice and considerable responsiveness in HEK293 cells expressing TLR3 [1], suggesting a specific recognition to poly(I:C) by TLR3. TLR3 signals mainly through a MyD88-independent pathway involving the TRIF/TICAM1 adapter protein that leads to the production of IFN-β and causes dendritic cells to mature [2].


1. Alexopoulou L. et al., 2001. Recognition of double-stranded RNA and activation of NF-kappaB by Toll-like receptor 3. Nature, 413(6857):732-8.
2. Yamamoto m. et al. 2002.
Cutting edge: A novel Toll/Il-1 receptor domain-containing adapter that preferentially activates the IFN-β promoter in the Toll-like receptor signaling. J Immunol, 169(12):6668-6672.

HEK-Blue™ hTLR3Human TLR3 / NF-κB / SEAP reporter HEK293 cells3-7 x 10e6 cells
HEK-Blue™ mTLR3Murine TLR3 / NF-κB / SEAP reporter HEK293 cells3-7 x 10e6 cells
HEK-Blue™ Null1 cellsNF-kappaB / SEAP parental cell line3-7 x 10e6 cells
HEK-Blue™ Null1-k cellsNF-kappaB / SEAP parental cell line3-7 x 10e6 cells
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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