Low-CpG pac

Synthetic puromycin resistance

ABOUT

Synthetic puromycin resistance gene in cloning plasmid

Resistance to the antibiotic puromycin is conferred by the Pac gene encoding a puromycin N-acetyl-transferase isolated from a Streptomyces strain.

The DNA sequence of this gene was modified by optimizing the codon usage and reducing CpG motifs to 16 without changing the amino acid sequence of the wild type protein.

The low-CpG Pac gene is provided in the bacterial plasmid pMOD2 which is selectable with Ampicillin.

To facilitate the excision and subcloning of the low-CpG Pac gene into another vector, this gene is flanked by a unique restriction site at the 5’ and 3’ end.

All products are for internal research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Gene
pac
Species
Bacteria
Plasmid backbone
pMOD2
Purification
Ion-exchange chromatography
Reconstitution buffer
Sterile Water
Quality control

Plasmid construct has been confirmed by restriction analysis and full-length open reading frame (ORF) sequencing Plasmid DNA was purified by ion exchange chromatography

CONTENTS

Contents

  • Product: 
    pMOD2-Puro
  • Cat code: 
    pmod2-puro
  • Quantity: 
    20 µg

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20°C

DOCUMENTS

Documents

pMOD2-Puro

Technical Data Sheet

Plasmid Sequence

Safety Data Sheet

Certificate of analysis

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