CpG-free hph

Resistance to the antibiotic hygromycin is conferred by the hph gene encoding a hygromycin B phosphotransferase isolated from an E. coli strain. The DNA sequence of this CpG-free gene was modified by optimizing the codon usage and deleting the CpG motifs without changing the amino acid sequence of the wild type protein.

The synthetic hph gene is provided in the bacterial plasmid pMOD2 which is selectable with Ampicillin. To facilitate the excision and subcloning of the synthetic hph gene into another vector, this gene is flanked by a unique restriction site at the 5’ and 3’ end.


Gene: CpG-free hph (hygromycin B phosphotransferase)
Description: Synthetic Hygromycin resistance gene
Backbone: pMOD2
Selection: Ampicillin

103 CpG in the native gene


• 20 µg of lyophilized pMOD2-Hygro plasmid DNA
• 4 pouches of E. coli Fast-Media® Amp (2 LB and 2 Agar)



Description pMOD2 bearing bacteria Synthetic hph Gene
Cat. Codepmod2-hygro
Unit Size20 µg
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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