pBOOST2 saIRF-3
| Product | Unit size | Cat. code | Docs. | Price | |
|---|---|---|---|---|---|
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pBOOST2-sahIRF3 pBOOST2 Human mutant IRF-3 |
Show product |
20 µg |
pbst2-sahirf3
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pBOOST2-samIRF3 pBOOST2 Murine mutant IRF-3 |
Show product |
20 µg |
pbst2-samirf3
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Genetic adjuvants for DNA vaccines with mutant IRF-3 gene | Increase Th1 response
pBOOST2-saIRF3 plasmids express a human or murine mutant interferon regulatory factor 3 (IRF-3) gene. IRF-3 has been shown to primarily increase Th1 T-cell responses [1].
A constitutively active form of IRF-3 was generated by creating a single point mutation of Ser396 to Asp. This super-activated IRF-3 presents a >10-fold enhanced transactivating potential over the wild-type IRF-3 for the IFN-α and IFN-β promoters [2].
pBOOST2-saIRF3 plasmids express the transgene under the control of the strong and ubiquitous EF-1α/HTLV composite promoter and are selectable in E. coli with Zeocin™.
References:
1. Sasaki S. et al., 2002. Regulation of DNA-raised immune responses by cotransfected interferon regulatory factors. J Virol.76(13):6652-9.
2. Servant MJ. et al., 2003. Identification of the minimal phosphoacceptor site required for in vivo activation of interferon regulatory factor 3 in response to virus and double-stranded RNA. J Biol Chem. 278(11):9441-7.
Specifications
Gene: super-activated IRF-3 gene
Species: Human or Murine
Backbone: pBOOST2
Selection: Zeocin
Th1 polarization
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- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperature
