Jurkat-Lucia™ NFAT CellsNEW

Jurkat-LuciaNFAT cells were derived from the human T lymphocyte-based Jurkat cell line by stable integration of an NFAT-inducible Lucia reporter construct. The Lucia gene, which encodes a secreted coelenterazine-utilizing luciferase, is driven by an ISG54 minimal promoter fused to six copies of the NFAT consensus transcriptional response element.

Jurkat-LuciaNFAT cells induce the activation of NFAT in response to PMA and ionomycin, and T-lymphocyte mitogens, such as concanavalin A (ConA) and phytohemagglutinin.

Jurkat-Lucia™ NFAT cells can be used for the study of NFAT activation following stimuli treatments by monitoring Lucia luciferase activity. Levels of Lucia luciferase are readily measurable in the cell culture supernatant when using QUANTI-Luc™, a Lucia luciferase detection reagent.

Jurkat-LuciaNFAT cells are resistant to the selective antibiotic Zeocin™.

Figures for this product

Jurkat-Lucia™ NFAT induction


Antibiotic resistance: Zeocin™

Guaranteed mycoplasma-free

Shipped on dry ice

These products are covered by a Limited Use License (See Terms and Conditions).


• 1 vial of Jurkat-Lucia™ NFAT cells (3-7 x 106 cells) in Freezing Medium
• 1 ml Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria and fungi
• 100 μl Zeocin™ (100 mg/ml)
• 1 pouch of QUANTI-Luc™

IMPORTANT: Cells are shipped frozen. If cells are not frozen upon arrival, contact InvivoGen immediately.


Jurkat-Lucia-NFAT pathway

NFAT (nuclear factor of activated T cells) proteins are a family of transcription factors that are important in the development and function of the immune system. In T cells, NFAT proteins regulate thymocyte development, T cell activation, differentiation and self-tolerance. Their activation is induced by the engagement of the T cell receptor (TCR) that is coupled to the calcium-signaling pathway. Calcium binds calmodulin and activates calcineurin, which in turn dephosphorylates NFAT proteins inducing their nuclear translocation and activation, and the transcription of specific responsive genes.


Jurkat-Lucia-NFAT cells PMA ConA graph


Jurkat-Lucia™ NFAT cells induced by PMA+ionomycinor by T-lymphocyte mitogen concanavalin A (ConA).


Jurkat-Lucia™ NFAT CellsNEW!

Description NFAT-Luc Reporter T lymphocytes
Cat. Codejktl-nfat
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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