STING Knockout IRF Reporter Cell Line

HEK-BlueISG-KO-STING cells were specifically designed to study the STING/TBK1/IRF3 signaling pathway. HEK-Blue™ ISG-KO-STING cells were generated from the HEK-Blue™ ISG cells through the stable knockout of the STING gene. The parental HEK-Blue™ ISG cells were derived from the PEAKrapid cell line (similar to ATCC® CRL-2828™) which itself was derived from the HEK293 cell line. Unlike their parental cell line, HEK-Blue™ ISG-KO-STING cells do not respond to cytosolic DNA, CDNs and xanthenone derivatives, such as DMXAA.

HEK-Blue™ ISG-KO-STING cells can detect bioactive type I IFNs through the activation of the JAK-STAT-IRF9 pathway. HEK-Blue™ ISG-KO-STING cells express a secreted embryonic alkaline phosphatase (SEAP) under the control of the IRF-inducible promoter comprised of five IFN-stimulated response elements (ISRE) fused to an ISG54 minimal promoter. Levels of SEAP in the supernatant can be easily determined with QUANTI- Blue™, a reagent that turns purple/blue in the presence of SEAP and by reading the OD at 620-655 nm.

Figures for this product

Figure 1A: IRF INDUCTION (SEAP reporter)Figure 1B: IRF INDUCTION (SEAP reporter)


Antibiotic resistance: G418Zeocin™

Quality control: STING knockout is verified by functional assays (see validation sheet) and DNA sequencing. These cells are guaranteed mycoplasma-free.

Growth Medium: DMEM, 4.5 g/l glucose, 10% (v/v) fetal bovine serum (FBS), 50 U/ml penicillin, 50 mg/ml streptomycin, 100 µg/ml Normocin™, 2 mM L-glutamine supplemented with Zeocin™ selective antibiotic only

Test Medium: DMEM, 4.5 g/l glucose, 10% (v/v) heat-inactivated FBS (30 min at 56°C), 50 U/ml penicillin, 50 µg/ml streptomycin, 100 µg/ml Normocin™, 2 mM L-glutamine

This product is covered by a Limited Use License (See Terms and Conditions).


1 vial containing 3-7 x 106 cells

100 μl Zeocin™ (100 mg/ml)

1 ml Normocin™ (50 mg/ml)

1 pouch of QUANTI-Blue™ (SEAP detection medium)

Shipped on dry ice


HEK-Blue ISG KO-STING stimulation pathway


STING (stimulator of interferon genes; also known as TMEM173, MITA, MPYS and ERIS) is essential for the interferon (IFN) response to cytosolic nucleic acids, such as microbial or self-DNA [1], and acts as a direct sensor of cyclic dinucleotides (CDNs) [2].

CDNs are important second messenger molecules in bacteria, affecting numerous responses of the prokaryotic cell. In mammalian cells, CDNs act as agonists of the innate immune response [3]. CDNs bind directly to and activate STING leading to a potent type I interferon (IFN) response.


1. Ishikawa H. & Barber G., 2008. STING is an endoplasmic reticulum adaptor that facilitates innate immune signalling. Nature 455, 674–678.

2. Burdette DL. et al., 2011. STING is a direct innate immune sensor of cyclic di-GMP. Nature 478(7370):515-8.

3. Wu J. et al., 2013. Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA. Science 339(6121):826-30.



Description HEK293 - STING Knockout IRF-reporter cells
Cat. Codehkb-kostg
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
please select your country
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
Copyrights © 2011-2016 InvivoGen. All Rights Reserved. Reproduction of any materials from this site is strictly forbidden without permission for commercial use. Nonprofit use for non-commercial research and educational purposes is permitted, citation should include the URL "www.invivogen.com".