B16-Blue™ IFN-α/β Cells
Murine Type I IFNs Sensor Cells
B16-Blue™ IFN-α/β cells allow the detection of bioactive murine type I IFNs by monitoring the activation of the JAK/STAT/ISGF3 pathway and/or IRF3 pathway.
They derive from the murine B16 melanoma cell line of C57BL/6 origin after stable transfection with a SEAP reporter gene under the control of the IFN-α/β-inducible ISG54 promoter enhanced by a multimeric ISRE.
Stimulation of B16-Blue™ IFN-α/β cells with murine IFN-α or IFN-β, or type I IFN inducers, such as the RLR ligands poly(I:C), poly(dA:dT) or 5’ppp-dsRNA delivered intracelluarly, triggers the production of SEAP by the activation of the IRF-inducible promoter. Levels of SEAP can be easily monitored using the detection medium QUANTI-Blue™.
Specifications
Antibiotic resistance: Zeocin™
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Shipped on dry ice
Detects murine type I interferons
• Detection range for mouse IFN-α: 102 - 104 IU/ml
• Detection range for mouse IFN-β: 102 - 104 IU/ml
Documents
Contents
1 vial containing 3-7 x 106 cells
100 μl Zeocin™ (100 mg/ml)
1 ml Normocin™ (50 mg/ml)
1 pouch of QUANTI-Blue™ (SEAP detection medium)
Details
Response of B16-Blue™ IFN-α/β cells following their stimulation with poly(I:C)-HMW, poly(dA:dT), 5’ppp-dsRNA, 5’ppp-dsRNA control delivered intracellularly using the transfection reagent, LyoVec™. Induction of type I IFNs was assessed by determining the levels of SEAP using QUANTI-Blue™.
Stimulation of B16-Blue™ IFN-α/β cells by recombinant human and murine IFN-α and IFN-β was assessed by measuring the levels of SEAP using QUANTI-Blue™.
ORDERING
B16-Blue™ IFNα/β cells
| Cat. Code | bb-ifnab |
|---|---|
| Unit Size | 3-7 x 10e6 |
| Price | For price or distributor address, please select your country |
