B16-Blue™ ISG Cells

Interferon Regulatory Factor-Inducible SEAP Reporter B16 Melanocytes

B16-Blue™ ISG cells allow the detection of bioactive murine type I and II IFNs by monitoring the activation of the JAK/STAT pathway.
B16-Blue™ ISG cells can also be used study the activation of the TBK1/IRF3 pathway by cytosolic DNA, dsRNA or CDNs.

B16-Blue™ ISG cells were derived from the murine B16 F1 melanoma cell line. They express the secreted embryonic alkaline phosphatase (SEAP) reporter gene under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.
Stimulation of B16- Blue™ ISG cells with IFNs, CDNs, such as cGAMP, or type I IFN inducers, such as transfected poly(dA:dT), triggers the activation of the I-ISG54 promoter and the production of SEAP.
Levels of SEAP in the supernatant can be easily determined using QUANTI- Blue™, a reagent that turns purple/blue in the presence of SEAP, by reading the OD at 620-655 nm.
B16-Blue™ ISG cells are resistant to Zeocin™.

Figures for this product

Response of B16-Blue ISG and B16-Blue ISG-KO-STING to CDNs and IFN-β


Murine IFN Detection range :
• mIFN-α: 10e2 - 10e4 IU/ml
• mIFN-β: 10e2 - 10e4 IU/ml
• mIFN-γ: 0.1 ng - 1 µg/ml

Antibiotic resistance: Zeocin™

Growth Medium: RPMI, 10% (v/v) fetal bovine serum (FBS), 50 U/ml penicillin, 50 µg/ml streptomycin, 100 µg/ml Normocin™, 2 mM L-glutamine

Quality control
Reporter activity is validated by stimulating the cells with murine IFN-α (mIFN-α), mIFN-β, mIFN-γ, and IRF3 activators, such as poly(dA:dT)/LyoVec™, c-di-GMP and cGAMP.
The cells are guaranteed mycoplasma-free.


1 vial containing 3-7 x 106 cells

100 μl Zeocin™ (100 mg/ml)

1 ml Normocin™ (50 mg/ml)

1 pouch of QUANTI-Blue™ (SEAP detection medium)

Shipped on dry ice


Recent articles using InvivoGen B16-Blue™ ISG Cells


B16-Blue™ ISG Cells

Description Murine B16 melanoma - IFNs reporter cells
Cat. Codebb-ifnabg
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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