3p-hpRNA is a 5’ triphosphate hairpin RNA that was generated by in vitro transcription of a sequence from influenza A (H1N1) virus, a single‑stranded negative‑sense RNA virus [1,2]. This 87 mer RNA oligonucleotide contains an uncapped 5’ triphosphate extremity and a double strand fragment. These structural features, which distinguish viral RNA from mammalian RNA, are recognized by retinoic acid-inducible gene I (RIG‑I), the founding member of the RIG-I like receptor (RLR) family [3,4]. RLRs are cytosolic sensors responsible for the detection of viral RNA. Upon activation, RIG-I recruits the adaptor mitochondrial antiviral signaling protein (MAVS, or IPS-1/VISA/Cardif) then triggers signaling cascades that lead to the production of type I interferons and pro-inflammatory cytokines [5]. Importantly, 3p-hpRNA is a specific agonist of RIG-I; it does not activate other dsRNA sensors such as TLR3 and MDA‑5. Of note, this ligand more potently induces RIG-I than the fully synthetic RIG-I ligand 5’ppp‑dsRNA.

Figures for this product

Evaluation of 3p-hpRNA in A549-Dual™ cellsEvaluation of 3p-hpRNA in RAW-Lucia™ ISG and HEK-Lucia™ RIG-I cells


Specificity: RIG-I agonist
Working Concentration:
 10 ng- 1 μg/ml
Molecular Weight: 28995 g/mol

Quality Control:
- The biological activity has been verified using A549-Dual™ cells.
- The absence of bacterial contamination, lipoproteins and endotoxins, hasbeen confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.


3p-hpRNA was prepared by in vitro transcription with T7 RNA polymerase. This sequence self-anneals to form secondary structures such as hairpin or panhandle conformations.


  • 25 μg 3p-hpRNA (5’ triphosphate hairpin RNA)
  • 1.5 ml sterile endotoxin-free water


  1. Rehwinkel J. et al., 2010. RIG-I detects viral genomic RNA during negative-strand RNA virus infection. Cell. 140:397-408.
  2. Liu G. et al., 2015. Influenza A Virus Panhandle Structure Is Directly Involved in RIG-I Activation and Interferon Induction. J Virol. 89(11):6067-79.
  3. Hornung V. et al., 2006. 5’-triphosphate RNA is the ligand for RIGI. Science. 314:994-7.
  4. Gebhardt A. et al., 2017. Discrimination of Self and Non-Self Ribonucleic Acids. Journal of Interferon & Cytokine Research 37: 184-97.
  5. Yoneyama M. et al., 2015. Viral RNA detection by RIG-I-like receptors. Curr Opin Immunol. 32:48-53.



Description 5’ triphosphate hairpin RNA; RIG-I ligand
Cat. Codetlrl-hprna
Unit Size25 µg
Price For price or distributor address,
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