NOD2
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pUNO1-hNOD2a pUNO1 bearing the human NOD2 gene, isoform 1 |
Show product |
20 µg |
puno1-hnod2a
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pUNO1-hNOD2a-DN pUNO1 bearing a dominant negative version of the human NOD2 gene |
Show product |
20 µg |
puno1-hnod2-dn
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pUNO1-mNOD2a pUNO1 bearing the mouse NOD2 gene, isoform 1 |
Show product |
20 µg |
puno1-mnod2a
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Expression-ready ORF Clones
Alias : NLRC2, CARD15, IBD1, BLAU
Nucleotide-binding oligomerization domain containing 2.
Caspase recruitment domain-containing protein 15.
Available versions:
- Wild type NOD2
- Dominant negative NOD2 (see the "Details" tab)
NOD2/CARD15 is an intracellular pattern-recognition proteins involved in bacterial detection which expression is restricted to monocytes [1]. NOD2 is a general sensor of bacterial peptidoglycan (PGN). It recognizes the minimal bioactive fragment of PGN, called muramyl dipeptide, also known as MDP, a motif found in all PGNs [2].
This recognition event triggers a pro-inflammatory signaling cascade regulated by NF-kB. Mutations in NOD2 have been recently related to increased susceptibility to Crohn’s disease (CD). NOD2 deficiency increases TLR2-mediated activation of NF-κB resulting in a pro-inflammatory cytokine bias which could contribute to the overwhelming inflammation seen in CD [3,4].
References:
1. Ogura Y. et al., 2001. Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-kappaB. J Biol Chem. 276(7):4812-8.
2. Girardin SE. et al., 2003. Nod2 is a general sensor of peptidoglycan through muramyl dipeptide (MDP) detection. J Biol Chem. 278(11):8869-72.
3. Watanabe T. et al., 2004. NOD2 is a negative regulator of Toll-like receptor 2-mediated T helper type 1 responses. Nat Immunol. 5(8):800-8.
4. Netea MG. et al., 2004. NOD2 mediates anti-inflammatory signals induced by TLR2 ligands: implications for Crohn’s disease. Eur J Immunol. 34(7):2052-9.
Specifications
Human NOD2 isoform 1 (pUNO1-hNOD2a)
ORF size : 3123 bp
Subclone : AgeI - NheI
Human NOD2-DN (pUNO1-hNOD2a-DN)
ORF size : 3123 bp
Subclone : AgeI - NheI
Mouse NOD2 isoform 1 (pUNO1-mNOD2a)
ORF size : 3063 bp
Subclone : BspHI - NheI
pUNO1 Contents
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.
Resuspended DNA should be stored at -20°C and is stable for up to 1 year.
Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.
Details
NOD2-DN
Dominant negative NOD2 was generated by a L145P mutation. This single amino-acid substitution in the CARD (caspase-recruitment domain) of the NOD2 gene inhibited the ability of the downstream factor RICK (also called RIP2 and CARDIAK) to activate NF-κB [1].
1. Tanabe T. et al. 2004. Regulatory regions and critical residues of NOD2 involved in muramyl dipeptide recognition EMBO J. 23(7): 1587-1597.
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