Coding sequence in expression plasmid

Alias: NLRC1, CARD4

Nucleotide-binding oligomerization domain containing 1
Caspase recruitment domain-containing protein 4

Available versions:

  • Wild type NOD1
  • Dominant negative NOD1 (see the "Details" tab)

Nucleotide-binding oligomerisation domain 1 (NOD1), also called caspase-recruitment domain (CARD)-4, is an intracellular pathogen-recognition molecule expressed ubiquitously in adult tissues. Upon bacterial invasion, NOD1 induces NF-κB activation pathways [1]. NOD1 recognizes a naturally occurring muropeptide of peptidoglycan (PGN) that presents a unique amino acid at its terminus called diaminopilemic acid (DAP) [2].
This amino acid is found mainly in the PGN of gram-negative bacteria designating NOD1 as a sensor of gram-negative bacteria. Recent studies show that signaling through NOD1 is able to activate NF-κB in cells infected with gram-negative bacteria that can bypass TLR activation [3].

This gene is available in pUNO1 expression plasmid selectable with Blasticidin.


Human NOD1 (pUNO1-hNOD1)

   Genbank: NM_006092.2
   ORF Size: 2862 bp
   Subclone: NcoI - EcoRI

Human NOD1-DN (pUNO1-hNOD1-DN)

   ORF Size: 2490 bp
   Subclone: NcoI - EcoRI

Mouse NOD1 (pUNO1-mNOD1)

   Genbank: NM_172729.3
   ORF Size: 2862 bp
   Subclone: NcoI - EcoRI


- 20 µg of lyophilized DNA.
- 4 pouches of E. coli Fast-Media® Blas (2 TB and 2 Agar)
- 1 ml blasticidin at 10 mg/ml



Dominant negative NOD1 was generated by a deletion of the centrally located nucleotide-binding site (NBS) domain (aa 127-518) [1].

1. Bertin JJ et al. 1999. Human CARD4 protein is a novel CED-4/Apaf-1 cell death family member that activates NF-κB. J Biol Chem. 274(19):12955-8


1. Inohara N. et al., 1999. Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-kappaB. J Biol Chem. 274(21):14560-7.
2. Girardin SE. et al., 2003. Nod1 detects a unique muropeptide from gram-negative bacterial peptidoglycan. Science. 300(5625):1584-7.
3. Kim JG. et al., 2004. Nod1 is an essential signal transducer in intestinal epithelial cells infected with bacteria that avoid recognition by toll-like receptors. Infect Immun. 72(3):1487-95.


Recent articles using InvivoGen NOD1



Description pUNO1 bearing the human NOD1 gene
Cat. Codepuno1-hnod1
Unit Size20 µg
Price For price or distributor address,
please select your country


Description pUNO1 bearing a dominant negative version of the human NOD1 gene
Cat. Codepuno1-hnod1-dn
Unit Size20 µg
Price For price or distributor address,
please select your country


Description pUNO1 bearing the mouse NOD1 gene
Cat. Codepuno1-mnod1
Unit Size20 µg
Price For price or distributor address,
please select your country
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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