pFUSE-CHIg-mG2b

pFUSE-CHIg-mG2b is a cloning plasmid that expresses the constant region of the mouse IgG2b heavy chain. It contains a multiple cloning site upstream of the constant region to enable cloning of the heavy chain variable region. This plasmid is designed for antibody generation when co-transfected into mammalian cells with the light chain variable region cloned into pFUSE-CLIg.

pFUSEss-CHIg-mG2b
additionally features the IL2 signal sequence to allow the secretion of recombinant antibodies generated using fragment antigen-binding (Fab) or single-chain variable fragment (scFv)  fragments selected from phage display libraries that lack a signal sequence.

Specifications

• pFUSE-CHIg-mG2b features the constant region of the mouse IgG2b heavy chain

pFUSEss-CHIg-mG2b features the constant region of the mouse IgG2b heavy chain and the IL2 signal sequence

Both plasmids are selectable with Zeocin™.

Contents

• 20 μg of lyophilized DNA
• 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)

ORDERING

pFUSE-CHIg-mG2b

Description Mouse IgG2b
Cat. Codepfuse-mchg2b
Unit Size20 µg
Price For price or distributor address,
please select your country

pFUSEss-CHIg-mG2b

Description Mouse IgG2b
Cat. Codepfusess-mchg2b
Unit Size20 µg
Price For price or distributor address,
please select your country
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