kappa or lambda light chain (CL)

pFUSE-CLIg expresses the constant region of the kappa or lambda light chain (CL) of human, mouse or rabbit origin. They contain a multiple cloning site (MCS) upstream of this constant region to enable the cloning of the variable (VL) region of a given antibody.

All pFUSE-CLIg plasmids are now available with or without the IL2 signal sequence. Addition of this signal sequence allows the secretion of recombinant IgGs generated using Fab or scFv fragments selected from phage display libraries that lack a signal sequence.

pFUSE-CLIg plasmids allow the generation of monoclonal antibodies when used in association with pFUSE-CHIg plasmids.
Antibodies generated using pFUSE-CLIg and pFUSE-CHIg can be purified from the supernatant of transfected cells using the appropriate Protein A, Protein G or Protein L affinity chromatography.


pFUSE2-CLIg-hKLight chain expression plasmid - Human kappa Human kappa (20 µg)
Human kappa with IL2ss (20 µg)
pFUSE2-CLIg-hL2Light chain expression plasmid - Human lambda2 Human lambda 2 (20 µg)
Human lambda 2 with IL2ss (20 µg)


pFUSE2-CLIg-mKLight chain expression plasmid - Murine kappa Mouse kappa (20 µg)
Mouse kappa with IL2ss (20 µg)
pFUSE2-CLIg-mL1Light chain expression plasmid - Murine lambda1 Mouse lambda 1 (20 µg)
Mouse lambda 1 with IL2ss (20 µg)
pFUSE2-CLIg-mL2Light chain expression plasmid - Murine lambda2 Mouse lambda 2 (20 µg)
Mouse lambda 2 with IL2ss (20 µg)


pFUSE2-CLIg-catKLight chain expression plasmid - Cat kappaCat kappa (20 µg)


pFUSE2-CLIg-dogKLight chain expression plasmid - Dog kappaDog kappa (20 µg)
pFUSE2-CLIg-dogLLight chain expression plasmid - Dog lambdaDog lambda (20 µg)


pFUSE2-CLIg-rK1 (rabbit)Light chain expression plasmid - Rabbit kappa 1 Rabbit kappa 1 (20 µg)
Rabbit kappa 1 with IL2ss (20 µg)
pFUSE2-CLIg-rK2 (rabbit)Light chain expression plasmid - Rabbit kappa 2 Rabbit kappa 2 (20 µg)
Rabbit kappa 2 with IL2ss (20 µg)


pFUSE2-CLIg-ratKLight chain expression plasmid - Rat kappa Rat kappa (20 µg)
Rat kappa with IL2ss (20 µg)
pFUSE2-CLIg-ratL1Light chain expression plasmid - Rat lambda 1 Rat lambda 1 (20 µg)
Rat lambda 1 with IL2ss (20 µg)

Rhesus monkey

pFUSE2-CLIg-rhKLight chain expression plasmid - Rhesus monkey kappa Rhesus monkey kappa (20 µg)
Rhesus monkey kappa with IL2ss (20 µg)
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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