pFUSE-CLIg

kappa or lambda light chain (CL)

pFUSE-CLIg expresses the constant region of the kappa or lambda light chain (CL) of human, mouse or rabbit origin. They contain a multiple cloning site (MCS) upstream of this constant region to enable the cloning of the variable (VL) region of a given antibody.

All pFUSE-CLIg plasmids are now available with or without the IL2 signal sequence. Addition of this signal sequence allows the secretion of recombinant IgGs generated using Fab or scFv fragments selected from phage display libraries that lack a signal sequence.

pFUSE-CLIg plasmids allow the generation of monoclonal antibodies when used in association with pFUSE-CHIg plasmids.
Antibodies generated using pFUSE-CLIg and pFUSE-CHIg can be purified from the supernatant of transfected cells using the appropriate Protein A, Protein G or Protein L affinity chromatography.

pFUSE-CLIg Products

pFUSE2-CLIg-hk Light chain constant region expression plasmid - Human kappa Human kappa (20 µg)
Human kappa with IL2ss (20 µg)
pFUSE2-CLIg-hl2 Light chain constant region expression plasmid - Human lambda 2 Human lambda 2 (20 µg)
Human lambda 2 with IL2ss (20 µg)
pFUSE2-CLIg-mk Light chain constant region expression plasmid - Murine kappa Mouse kappa (20 µg)
Mouse kappa with IL2ss (20 µg)
pFUSE2-CLIg-ml1 Light chain constant region expression plasmid - Murine lambda 1 Mouse lambda 1 (20 µg)
Mouse lambda 1 with IL2ss (20 µg)
pFUSE2-CLIg-ml2 Light chain constant region expression plasmid - Murine lambda 2 Mouse lambda 2 (20 µg)
Mouse lambda 2 with IL2ss (20 µg)
pFUSE2-CLIg-rk1 Light chain constant region expression plasmid - Rabbit kappa 1 Rabbit kappa 1 (20 µg)
Rabbit kappa 1 with IL2ss (20 µg)
pFUSE2-CLIg-rk2 Light chain constant region expression plasmid - Rabbit kappa 2 Rabbit kappa 2 (20 µg)
Rabbit kappa 2 with IL2ss (20 µg)
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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