Single-stranded RNAs

Single-stranded RNA (ssRNA) has been identified as the natural ligand of TLR7 and TLR8 [1, 2]. ssRNA derived from HIV-1 or the influenza virus were shown to induce the production of proinflammatory cytokines in pDC [3, 4]. This induction was reproduced using polyU or GU-rich (ssRNA40) ODNs complexed with cationic lipids to protect them from degradation. Upon stimulation with ssRNA, murine TLR7 and human TLR8 induced the activation of NF-κB, whereas human TLR7 and murine TLR8 failed, implying a species specificity difference in ssRNA recognition.

All ssRNAs/ORNs have been tested using HEK-Blue™ TLR7 and HEK-Blue™ TLR8 Cells to confirm their specificity.

1. Diebold SS. et al., 2004. Innate antiviral responses by means of TLR7-mediated recognition of single-stranded RNA. Science. 5;303(5663):1529-31
2. Heil F. et al., 2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. Science. 5;303(5663):1526-9.
3. Alter G. et al., 2007. Single-Stranded RNA Derived from HIV-1 Serves as a Potent Activator of NK Cells. J Immunol. 178:7658-7666.
4. Hornung V. et al., 2005. Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7.Nat Med. 11(3):263-70.

ORN06/LyoVec™TLR8 Agonist - ssRNA with 6 UUGU repeats / LyoVec™4 x 25 µg
ssPolyU NakedTLR8 Agonist - RNA homopolymer 10 mg
100 mg (10 x 10 mg)
ssPolyU/LyoVec™TLR8 Agonist - RNA homopolymer / LyoVec™4 x 25 µg
ssRNA40/LyoVec™TLR8 Agonist - HIV-1 LTR-derived ssRNA / LyoVec™4 x 25 µg
ssRNA41/LyoVec™TLR8 Agonist - ssRNA40 control / LyoVec™4 x 25 µg
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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