Reporter Cell Lines
InvivoGen provides a large choice of reporter cell lines designed to provide a simple, rapid and reliable method to monitor the activation of intracellular signaling pathways induced by microbial molecules or cytokines. These engineered cell lines, named Blue™ Reporter Cells stably express an inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene. Exposure to a stimulus induces the pathway modulating SEAP activity that can be readily assessed qualitatively and quantitatively using HEK-Blue™ Detection or QUANTI-Blue™. These SEAP detection media turn blue in the presence of the reporter protein.
Blue™ Reporter Cells comprise cell lines of different species and origins:
B16-Blue™ cells - Murine B16 melanoma expressing an IFN-inducible SEAP reporter gene
HEK-Blue™ cells - Engineered human embryonic kidney (HEK)293 cells expressing a SEAP reporter gene under the control of various transcription factors - HEK-Blue™ cells comprise three families: HEK-Blue™ TLR cells, HEK-Blue™ NOD cells and HEK-Blue™ Cytokine cells.
MEF-Blue™ cells - Murine embryonic fibroblasts from C3H or C57BL/6 mouse embryos expressing an NF-κB/AP-1- or ISRE/NF-κB/AP-1-inducible SEAP reporter gene
Ramos-Blue™ cells - Human B lymphocytes expressing an NF-κB/AP-1-inducible SEAP reporter gene
RAW-Blue™ cells - Murine RAW 264.7 macrophages expressing an NF-κB/AP-1-inducible SEAP reporter gene
THP1-XBlue™ cells - Human THP-1 monocytes expressing an NF-κB/AP-1-inducible SEAP reporter gene
