HMGB1-Lucia
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pSELECT-hHMGB1-Lucia hHMGB1::Lucia fusion gene |
Show product |
20 µg |
psetz-hhmgbluc
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pSELECT-mHMGB1-Lucia mHMGB1::Lucia fusion gene |
Show product |
20 µg |
psetz-mhmgbluc
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Expression-ready ORF Clones
pSELECT-HMGB1-Lucia are mammalian vectors designed for expression of the human or murine HMGB1::Lucia fusion genes. These plasmids are selectable in bacteria and mammalian cells with Zeocin™.
pSELECT-HMGB1::Lucia plasmids contain two expression cassettes.
- The first one delivers strong and constitutive expression of HMGB1::Lucia.
- The second one drives the zeocin-resistance gene (Sh ble) that allows the selection of stable transfectants (and selection in bacteria).
Each cassette terminates with a strong polyadenylation signal (polyA) thus preventing any transcription interference. In order to obtain enough copies for HMGB1::Lucia expression in mammalian cells, we recommend to use this plasmid for viral gene transfer.
Expression of HMGB1::Lucia in cells equipped for inflammasome mediated-pyroptosis and/or necroptosis allows monitoring of these forms of necrotic cell death.
Back to the topSpecifications
Selection: Zeocin™
Gene: HMGB1::Lucia fusion gene
Promoter: hEF1-HTLV
Back to the topContents
- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperature