ODN TTAGGG (A151), also known simply as A151, is a synthetic oligonucleotide (ODN) containing the immunosuppressive motif TTAGGG.

Numerous studies have confirmed the existence of DNA sequences that can neutralize the stimulatory effect of CpG-containing oligonucleotides (CpG ODNs) [1-3]. These sequences are characterized by three consecutive Gs downstream of a C or A. The most potent inhibitory sequences are (TTAGGG)4 found in mammalian telomeres [2]. Indeed, ODN TTAGGG (A151) which comprises the suppressive (TTAGGG)4 motif has been shown to block TLR9 signaling [3], prevent STAT1 and STAT4 phosphorylation, and downregulate the production of pro-inflammatory cytokines [3, 4]. In addition, pretreatment of macrophages and dendritic cells with this ODN inhibited the interferon response to cytosolic DNA [3].


Specificity: human TLR9 antagonist

Working concentration: 100 nM - 10 µM

Solubility:  2.5 mg/ml in water

ODN TTAGGG sequence
5’- tt agg gtt agg gtt agg gtt agg g -3’ (24 mer)
Note: Bases are phosphorothioate (nuclease resistant).

Quality control:
- TLR9 activity has been tested using HEK-Blue™ TLR9 cells.
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.


ODN TTAGGG (A151) is provided lyophilized and is available in two quantities.

- 200 μg (25.2 nmol) lyophilized ODN TTAGGG (A151)
- 1.5 ml endotoxin-free water

- 1 mg (126 nmol) lyophilized ODN TTAGGG (A151)
- 1.5 ml endotoxin-free water

ODN TTAGGG (A151) is shipped at room temperature. Upon receipt, store at -20 °C.


1. Krieg AM. et al., 1998. Sequence motifs in adenoviral DNA block immune activation by stimulatory CpG motifs. Proc Natl Acad Sci USA. 95(21): 12631-6.
2. Gursel I. et al., 2003. Repetitive elements in mammalian telomeres suppress bacterial DNA-induced immune activation. J Immunol. 171(3):1393-400.
3. Kaminski J. et al., 2013. Synthetic oligodeoxynucleotides containing suppressive TTAGGG motifs inhibit AIM2 inflammasome activation. J Immunol. 191(7):3876-83.
4. Shirota H. et al., 2005. Suppressive oligodeoxynucleotides protect mice from lethal endotoxic shock. J Immunol. 174(8):4579-83.


Recent articles using InvivoGen ODN TTAGGG (A151)



Description TLR9 antagonist - Human preferred
Cat. Codetlrl-ttag151
Unit Size200 µg
Price For price or distributor address,
please select your country


Description TLR9 antagonist - Human preferred
Cat. Codetlrl-ttag151-1
Unit Size1 mg
Price For price or distributor address,
please select your country
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