Recombinant human & murine IL-16

Recombinant human and murine interleukin 16 (carrier-free)

InvivoGen offers untagged recombinant human (h) and mouse (m) interleukin 16 (IL-16) in their mature and intracellular form:

– Recombinant hIL-16: [S1212-S1332] C-terminal region of human pro-IL-16
– Recombinant mIL-16: [S1205-S1322] C-terminal region of mouse pro-IL16

IL-16 is a pleiotropic pro-inflammatory cytokine produced by a variety of immune (e.g. T cells, eosinophils, neutrophils, dendritic cells) and non-immune (e.g. fibroblasts, epithelial, neuronal) cells [1]. It is synthesized as a large (~80 kDa) inactive precursor protein (pro-IL-16) stored inside the cell prior to activation [2]. Caspase-3 mediates pro-IL-16 proteolytic cleavage and release of two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal region (~14 kDa), while the N-terminal product may play a role in cell cycle control [3,4].

The precise secretion mechanism of mature IL-16 is still unclear. IL-16 amino acid sequence does not contain a secretory signal peptide, suggesting an unconventional secretion pathway [2].

 More details

InvivoGen’s recombinant hIL-16 and mIL-16 were engineered from cDNA sequences with no signal sequence. The cytokines were purified from transfected HEK cell lysates using an anti-IL-16 monoclonal antibody. The preparation contains a majority of monomeric mature IL-16 as determined by gel filtration (see figures).

Importantly, InvivoGen’s recombinant hIL-16 and mIL-16 are guaranteed free of bacterial contaminations (e.g. lipoproteins and endotoxins) which could interfere with IL-16 specific signaling in TLR2- and TLR4-expressing target cells (see figures).

Key features:

• Produced in HEK cells
• High-quality: purity ≥ 95% and endotoxin level < 0.1 EU/µg
• Guaranteed free of bacterial contamination (e.g. lipoproteins and endotoxins) as confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells. 
   

Applications:

• Cellular assays
• ELISA
• Western-Blot
• In vivo assays in mice (for mIL-16)

Human and murine IL-16 provided by InvivoGen are for research use only.

References:

1. Cruikshank, W.W., H. Kornfeld, and D.M. Center, 2000. Interleukin-16. J Leukoc Biol. 67(6): p. 757-66.
2. Cruikshank, W.W., et al., 1994. Molecular and functional analysis of a lymphocyte chemoattractant factor: association of biologic function with CD4 expression. Proc Natl Acad Sci U S A. 91(11): p. 5109-13.
3. Baier, M., et al., 1997. Molecular cloning, sequence, expression, and processing of the interleukin 16 precursor. Proc Natl Acad Sci U S A. 94(10): p. 5273-7.
4. Zhang, Y., et al., 1998. Processing and activation of pro-interleukin-16 by caspase-3. J Biol Chem. 273(2): p. 1144-9.

Figures

SDS PAGE and WES analysis of the recombinant human (h) and murine (m)IL-16 proteins. (A) 0.5 μg of hIL-16 or mIL-16 was loaded on a 12% Mini-PROTEAN® TGX Stain-Free™ Precast Gel (Bio-Rad). Detection was performed as per the manufacturer’s instructions. A band was detected at ~17 kDa and ~14 kDa for hIL-16 and mIL-16, respectively. (B) 4 μg of  hIL-16 or mIL-16 was analyzed by WES using InvivoGen’s anti-hIL-16 hIgG1 and anti-mIL-16 mIgG1e3 antibodies, followed by an HRP-conjugated secondary antibody. A band was detected at ~23 kDa and ~21 kDa for hIL-16 and mIL-16, respectively.

Detection of the recombinant murine (m)IL-16 protein by ELISA. Coated ELISA plates with increasing concentrations of mIL-16 were incubated with 2 µg/ml of Anti-mIL-16-mIgG1e3 mAb (red curve) or Anti-β-Gal-mIgG1e3 control antibody (grey curve) for 2h, followed by an HRP-conjugated anti-mouse secondary antibody (1/1000 dilution). Data are shown as optical density (OD) at 480 nm (mean + SEM).

Detection of the recombinant human (h)IL-16 protein by ELISA.  Coated ELISA plates with increasing concentrations of hIL-16 were incubated with 2 µg/ml of Anti-hIL-16-hIgG1 mAb (red curve) or Anti-β-Gal-hIgG1 control antibody (grey curve) for 2h, followed by an HRP-conjugated anti-human secondary antibody (1/1000 dilution). Data are shown as optical density (OD) at 480 nm (mean + SEM).

Detection of TLR2 and TLR4 agonists in murine (m)IL-16. HEK-Blue™ hTLR2 (A) or HEK-Blue™ hTLR4 (B) were cultured with increasing concentration of recombinant mIL-16 produced in HEK cells (InvivoGen) or E. coli (Competitor). After 24h, the level of NF-κB-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene was assessed using the QUANTI-Blue™ Solution detection reagent. Optical density was read at 630 nm. Data are shown as mean + SEM.

Detection of TLR2 and TLR4 agonists in human(h)IL-16. HEK-Blue™ hTLR2 (A) or HEK-Blue™ hTLR4 (B) were cultured with increasing concentration of recombinant hIL-16 produced in HEK cells (InvivoGen) or E. coli (Competitor). After 24h, the level of NF-κB-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene was assessed using the QUANTI-Blue™ Solution detection reagent. Optical density was read at 630 nm. Data are shown as mean+SEM.

Specifications

Recombinant human IL-16

Source: Mammalian; Human embryonic kidney (HEK) cells

Uniprot ID: Q14005 

Alternate name: Lymphocyte chemoattractant factor (LCF)

Protein sequence: [S1212-S1332] 

Formulation: 20 mM Sodium Phosphate, 150 mM NaCl, and 5% saccharose buffer solution (pH 7)

Molecular mass: ~17 kDa (SDS PAGE), ~23 kDa (WES)

Monomer frequency: predominant (gel filtration)

Solubility: 100 μg/ml in water

Quality control:

•    Purity: ≥95% (SDS-PAGE)
•    Endotoxin level: ≤ 0.1 EU/μg (measurement by kinetic chromogenic LAL assay)
•    The absence of bacterial contamination (e.g. lipoproteins and endotoxins) is confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.

Recombinant murine IL-16

Source: Mammalian; Human embryonic kidney (HEK) cells

Uniprot ID: O54824

Alternate name: Lymphocyte chemoattractant factor (LCF)

Protein sequence: [S1205-S1322] 

Formulation: 20 mM Sodium Phosphate, 150 mM NaCl, and 5% saccharose buffer solution (pH 7)

Molecular mass: ~14 kDa (SDS PAGE), ~21 kDa (WES)

Monomer frequency: predominant (gel filtration)

Solubility: 100 μg/ml in water

Quality control:

•    Purity: ≥95% (SDS-PAGE)
•    Endotoxin level: ≤ 0.1 EU/μg (measurement by kinetic chromogenic LAL assay)
•    The absence of bacterial contamination (e.g. lipoproteins and endotoxins) is confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.

Contents

Note: Each product is sold separately.

Recombinant human IL-16

• 50 μg of lyophilized recombinant hIL-16.
• 1.5 ml endotoxin-free water.

 Recombinant hIL-16 is shipped at room temperature.

 Upon receipt, it should be stored at -20°C.

Recombinant murine IL-16

• 50 μg (rcyc-mil16) OR 500 µg (rcyc-mil16-10) of lyophilized recombinant mIL-16.
• 1.5 ml endotoxin-free water.

 Recombinant mIL-16 is shipped at room temperature.

 Upon receipt, it should be stored at -20°C.

Details

IL-16: a pleiotropic cytokine

Interleukin 16 (IL-16, initially named lymphocyte chemoattractant factor (LCF)) is a pro-inflammatory cytokine playing an important role in modulating T cell activation, chemotaxis, and proliferation [1]. It has also been classified as an alarmin, conveying an endogenous danger signal when released by stressed or necrotic cells [2]. IL-16 initially gained attention due to its activating functions in CD4+ T cells, as well as its contribution to inhibiting HIV replication [3]. It has since been also associated with the development of several cancers as well as the exacerbation of infectious, immune-mediated, and autoimmune inflammatory disorders [4, 5]. More recently, high plasmatic levels of IL-16 were found to correlate with COVID-19 severity [6, 7].

IL-16 production

IL-16 is produced by a variety of immune (e.g. T cells, eosinophils, neutrophils, dendritic cells) and non-immune (e.g. fibroblasts, epithelial, neuronal) cells [1, 8, 9]. It is synthesized as a large (~80 kDa) inactive precursor protein (pro-IL-16) stored inside the cell prior to activation [10]. Caspase-3 mediates the pro-IL-16 proteolytic cleavage and the release of two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal region (Cter-IL-16 or IL-16C, ~14 kDa), while the N-terminal product may play a role in cell cycle control [11, 12]. The exact stimuli that trigger pro-IL-16 processing by caspase-3 are not yet fully elucidated and differ with the cell type. Among human T lymphocytes, resting CD8+ T cells contain constitutively active caspase-3 and therefore, stored intra-cytoplasmic bioactive IL-16C. On the contrary, activation of caspase-3 in CD4+ T cells requires TCR stimulation [13]. In human neutrophils, pro-IL-16 is processed by caspase-3 when the cells undergo apoptosis [9]. In a model of human lung epithelial cells, SARS-CoV-2 infection triggers the release of bioactive IL-16C, possibly as a consequence of the NLRP1 inflammasome activation [7].

Bioactive IL-16C release

The precise secretion mechanism of mature IL-16 is still unclear. IL-16 amino acid sequence does not contain a secretory signal peptide, suggesting an unconventional secretion pathway. Multiple unconventional secretion pathways may be at play, depending on the cell type. In human neutrophils, bioactive IL-16C was shown to be released passively upon secondary necrosis [9, 10]. In lung epithelial cells, it may be released through Gasdermin E pores forming at the cell membrane in response to the NLRP1 inflammasome activation [7].

IL-16 sensing and biological functions

Bioactive IL-16C sensing and functions

IL-16 was initially described as a T cell-specific chemoattractant factor that mediates its functions upon binding to the CD4 transmembrane molecule [14], and more precisely to the D4 domain of CD4 [15]. Several lines of evidence indicate that IL-16C exerts its biological activity as homo-tetramers [10, 15, 16]. In CD4+ T cells, IL-16 triggers cell cycle progression and chemotaxis. The intracellular molecules thought to conduct the signal from the surface CD4 to the cytoskeleton for motility include PI3K (Phosphoinositide 3-kinase) and PLCγ (phospholipase C gamma) [17].
IL-16 sensing and signaling outcomes differ among cell types. Indeed, in a variety of myeloid cells (e.g. monocytes, macrophages, eosinophils, mast cells) which express CD4, this receptor seems to be dispensable for IL-16-mediated functions [18, 19]. Moreover, IL-16-stimulated monocytes, unlike T cells, produce pro-inflammatory cytokines, such as IL-6 and TNF-α [19].
Among the possible alternate receptors for IL-16, the tetraspanin CD9 surface molecule has been shown to participate in the IL-16-mediated chemotaxis and activation of mast cells [20]. Likewise, CD9 has been suggested as a receptor for IL-16 in human lung cells using the A549 cellular model [21]. Of note, IL-16 may also trigger chemotaxis indirectly, by inducing the expression of other chemokines. Indeed, IL-16 can act on lung epithelial cells to induce the upregulation of neutrophil-attracting chemokines, such as CXCL10 [22].

References:

1. Cruikshank, W.W., et al., 2000. Interleukin-16. J Leukoc Biol. 67(6): p. 757-66.
2. Rider, P., et al., 2017. Alarmins: Feel the Stress. The Journal of Immunology. 198(4): p. 1395-1402.
3. Amiel, C., et al., 1999. Interleukin-16 (IL-16) inhibits human immunodeficiency virus replication in cells from infected subjects, and serum IL-16 levels drop with disease progression. J Infect Dis. 179(1): p. 83-91.
4. Glass, W.G.,  et al., 2006. Not-so-sweet sixteen: the role of IL-16 in infectious and immune-mediated inflammatory diseases. J Interferon Cytokine Res. 26(8): p. 511-20.
5. Richmond, J., et al., 2014. Regulation of Cellular Processes by Interleukin-16 in Homeostasis and Cancer. Journal of Cellular Physiology. 229(2): p. 139-147.
6. Lucas, C., et al., 2020. Longitudinal analyses reveal immunological misfiring in severe COVID-19. Nature. 584(7821): p. 463-469.
7. Planès, R., et al., 2022. Human NLRP1 is a sensor of pathogenic coronavirus 3CL proteases in lung epithelial cells. Molecular Cell. 82(13): p. 2385-2400.e9.
8. Wilson, K.C., et al., 2004. The effect of interleukin-16 and its precursor on T lymphocyte activation and growth. Growth Factors. 22(2): p. 97-104.
9. Roth, S., et al., 2015. Secondary necrotic neutrophils release interleukin-16C and macrophage migration inhibitory factor from stores in the cytosol. Cell Death Discov. 1: p. 15056.
10. Cruikshank, W.W., et al., 1994. Molecular and functional analysis of a lymphocyte chemoattractant factor: association of biologic function with CD4 expression. Proc Natl Acad Sci U S A. 91(11): p. 5109-13.
11. Baier, M., et al., 1997. Molecular cloning, sequence, expression, and processing of the interleukin 16 precursor. Proc Natl Acad Sci U S A. 94(10): p. 5273-7.
12. Zhang, Y., et al., 1998. Processing and activation of pro-interleukin-16 by caspase-3. J Biol Chem. 273(2): p. 1144-9.
13. Wu, D.M., et al., 1999. Processing and release of IL-16 from CD4+ but not CD8+ T cells is activation dependent. J Immunol. 162(3): p. 1287-93.
14. Center, D.M., et al., 1996. Interleukin 16 and its function as a CD4 ligand. Immunology Today, 17(10): p. 476-481.
15. Liu, Y., et al., 1999. Identification of a CD4 domain required for interleukin-16 binding and lymphocyte activation. J Biol Chem. 274(33): p. 23387-95.
16. Keane, J., et al., 1998. Conservation of structure and function between human and murine IL-16. J Immunol. 160(12): p. 5945-54.
17. Cruikshank, W.W., et al., 1998. Signaling and Functional Properties of lnterleukin-16. International Reviews of Immunology. 16(5-6): p. 523-540.
18. Mathy, N.L., et al., 2000. Cutting edge: CD4 is not required for the functional activity of IL-16. J Immunol. 164(9): p. 4429-32.
19. Mathy, N.L., et al., 2000. Interleukin-16 stimulates the expression and production of pro-inflammatory cytokines by human monocytes. Immunology. 100(1): p. 63-9.
20. Qi, J.C., et al., 2006. Human and mouse mast cells use the tetraspanin CD9 as an alternate interleukin-16 receptor. Blood. 107(1): p. 135-42.
21. Blake, D.J., et al., 2018. Ablation of the CD9 receptor in human lung cancer cells using CRISPR/Cas alters migration to chemoattractants including IL-16. Cytokine. 111: p. 567-570.
22. Smith, S., et al., 2018. IL-16/miR-125a axis controls neutrophil recruitment in pristane-induced lung inflammation. JCI Insight. 3(15).