HEK-Blue™ hTLR2

HEK-Blue™-hTLR2 cells were obtained by co-transfection of the human TLR2 and SEAP (secreted embryonic alkaline phosphatase) genes into HEK293 cells.
The SEAP reporter gene is placed under the control of the IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites. Additionally, the CD14 co-receptor gene was transfected into these cells to enhance the TLR2 response. Stimulation with a TLR2 ligand activates NF-κB and AP-1 which induce the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.

The parental cell line of HEK-Blue™ hTLR2 cells is HEK-Blue™ Null1 cells.

Figures for this product

Response of HEK-Blue™ hTLR2 cells to TLR and NOD agonistsResponse of HEK-Blue™ hTLR2 to TLR2 agonistsTLR2 agonists dose responseNeutralization of TLR1/2 dose responseNeutralization of TLR2/6 dose response


Antibiotic resistance: blasticidin, hygromycin, Zeocin™

Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

Shipped on dry ice


1 vial containing 3-7 x 106 cells

2 x 1 ml 250X HEK-Blue™ Selection

1 ml Normocin™ (50 mg/ml)

1 pouch of HEK-Blue™ Detection (cell culture medium for real-time detection of SEAP)


HEK-Blue™ hTLR2 cells

Description human TLR2-expressing HEK 293 cells
Cat. Codehkb-htlr2
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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