Toll-like receptor 5 (TLR5) is a type-1 transmembrane receptor comprising an N-terminal extracellular leucine rich repeat domain and a C-terminal intracellular TIR signaling domain.

The soluble TLR5 receptor, hTLR5-Fc, was generated by fusing the N-terminal extracellular domain of human TLR5 (aa 21-639) to the N-terminus of an engineered Fc region of human IgG1 with a 2 amino acid linker.

The hTLR5-hFc fusion has an apparent molecular weight of 110 kDa on SDS-PAGE.

Fc-hTLR5 is expressed in CHO cells and purified by protein G affinity chromatography.


Applications: Neutralization, receptor binding assays

Formulation: 0.2 µm filtered solution in phosphate buffer with glycine, saccharose and stabilizing agents


- 50 µg lyophilized Fc-hTLR5

Product is shipped at room temperature.
Store lyophilized product at -20°C. Product is stable for at least 1 year.


Neutralization activity of hTLR5-Fc

Neutralization activity of hTLR5-Fc:

Increasing concentrations of hTLR5-Fc, anti-FLA-ST (antibody against S. typhimurium flagellin) or anti-hTLR5 (antibody against human TLR5) were pre-incubated with 5 µg FLA-ST (S. typhimurium flagellin) prior to the addition of HEK-Blue™ hTLR5 cells, which express the human TLR5 gene and an NF-kB-inducible SEAP gene.
After 24h incubation, TLR5-induced NF-kB activation was assessed by measuring the levels of SEAP using the QUANTI-Blue™ assay.


Recent articles using InvivoGen hTLR5-Fc



Description Soluble ectodomain of TLR5
Cat. Codefc-htlr5
Unit Size50 µg
Price For price or distributor address,
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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