Ramos B lymphocytes
Ramos-Blue™ cells derive from a human Burkitt’s lymphoma which is negative for Epstein Barr virus. They have the characteristics of B lymphocytes and are routinely used as a model of B lymphocytes and for apoptosis studies. Ramos-Blue™ cells stably express an NF-κB/AP-1-inducible SEAP (secreted embryonic alkaline phosphate) reporter construct.
Ramos-Blue™ cells express several pattern recognition receptors, including TLR3, TLR7 and TLR9. The Ramos-Blue™ cell line was isolated for its ability to respond to CpG ODNs (TLR9 ligands).
Two cell lines are available:
- Ramos-Blue™ cells can be used to study the NF-κB and AP-1 signaling pathways in B lymphocytes, and in particular the TLR3, TLR7, TLR9 and NOD1 signaling pathways.
- Ramos-Blue™-defMyD are MyD88-deficient cells. These cells are unable to respond to the activation of MyD88-dependent receptors, such as TLR7 and TLR9, but are still responsive to TLR3 which is MyD88-independent.