E.coli Competent Cells
InvivoGen provides two different E. coli competent strains:
• GT115 cells are specifically designed for cloning and propagation of plasmids containing hairpin structures and the R6K gamma origin of replication, such as pCpG-siRNA plasmids
• GT116 cells are specifically designed for cloning and propagation of shRNA-expressing plasmids which contain hairpin structures, such as psiRNA plasmids.
The strains are made chemically competent by an optimized procedure followed by transformation efficiency verification and provided frozen (ChemiComp) or lyophilized (LyoComp).