Antibody Isotype Collection
Immunoglobulins (Ig) are divided in isotypes: nine in humans (IgG1, IgG2, IgG3, IgG4, IgM, IgA1, IgA2, IgD, IgE) and eight in mice (IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgD, IgE).
Each isotype displays distinct structural and effector properties, such as complement-dependent cytotoxicity (CDC) and antibody-dependent cell-mediated cytotoxicity (ADCC), as presented in the table below. These properties are key features in choosing the backbone for a therapeutic antibody.
To help you decide which Ig isotype is the most suitable for your application, InvivoGen provides two well-known monoclonal antibodies, anti-hCD20 (rituximab) and anti-hTNF-α (adalimumab), available in the most common human and murine isotypes. These antibody isotype collections will help you study the effector functions between different isotypes or between the same isotype of two different species.
| Name | Types | Description |
| IgG | 4 |
Major Ig in serum, placental transfer CDC (hIgG3>hIgG1>hIgG2>hIgG4; mIgG2a>mIgG1) ADCC (hIgG1≥hIgG3>hIgG2≥IgG4; mIgG2a>mIgG1) |
| IgM | 1 | Third most common serum Ig, first Ig to be made Good CDC, some ADCC |
| IgA | 2 | Major class in secretions, second most common serum Ig monomer in serum, dimer in secretions No CDC, some ADCC |
| IgE | 1 | Least common serum Ig, involved in allergic reaction Strong binding to Fc receptors on basophils, no CDC |
Antibody Isotype Collection
| Anti-hCD20 isotype collection | Collection of antibodies, variable region of rituximab (anti-CD20) |
|---|---|
| Anti-hTNF-α isotype collection | Collection of antibodies, variable region of adalimumab (anti-hTNF-α) |