Immunoglobulin G - Review


Antibody structureImmunoglobulin G (IgG) antibodies are large molecules composed of two heavy chains γ and two light chains, either κ or λ.

They can be separated in two regions: the Fab (fragment-antigen binding) that contains the variable domain responsible for the antibody specificity, and the Fc (fragment crystalline) that binds specific proteins to induce immune responses such as opsonization and cell lysis.

The IgG class is divided in four isotypes: IgG1, IgG2, IgG3 and IgG4 in humans, and IgG1, IgG2a, IgG2b and IgG3 in mice. They share more than 95% homology in the amino acid sequences of the Fc regions but show major differences in the amino acid composition and structure of the hinge region.

The Fc region mediates effector functions, such as antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC).
In ADCC, the Fc region of an antibody binds to Fc receptors (FcγRs) on the surface of immune effector cells such as natural killers and macrophages, leading to the phagocytosis or lysis of the targeted cells. In CDC, the antibodies kill the targeted cells by triggering the complement cascade at the cell surface. I

IgG isoforms exert different levels of effector functions increasing in the order of IgG4

Antibody Effector Activities and Affinities

Isotype Species ADCC CDC Protein A binding Protein G binding Protein L binding
IgG1 Human +++
+++ ++++ ++++ ++++
IgG2 Human +/-
+ ++++ ++++ ++++
IgG3 Human +++
++++ - ++++ ++++
IgG4 Human +/-
- ++++ ++++ ++++
IgG1 Mouse -
+/- + ++++ ++++
IgG2a Mouse +++
+++ ++++ ++++ ++++
IgG2b Mouse +++
+++ +++ +++ ++++
IgG3 Mouse +++
+ ++ +++ ++++
IgG Rabbit N/A
+++ ++++ +++ +
IgG2b Rat ++
++ - ++ ++++


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