Poly(I:C) HMW

Polyinosinic-polycytidylic acid (usually abbreviated as poly(I:C) or poly(rI):poly(rC)) is a synthetic analog of double-stranded RNA (dsRNA), a molecular pattern associated with viral infection. Poly(I:C) activates the antiviral pattern recognition receptors TLR3, RIG-I/MDA5 and PKR, thereby inducing signaling via multiple inflammatory pathways, including NF-kB and IRF.

High Molecular Weight Poly(I:C) comprises long strands of inosine poly(I) homopolymer annealed to strands of cytidine poly(C) homopolymer. The average size of Poly(I:C) HMW is from 1.5 kb to 8 kb.

InvivoGen poly(I:C) HMW is a highly referenced as potent inducer of IFN both in vitro and in vivo.

Poly(I:C) HMW is functionally validated for TLR3 potency using HEK-Blue™ TLR3 Cells. It is tested in TLR2 and TLR4 cellular assays to confirm the absence of microbial contaminants (e.g. endotoxin or bacterial-membrane components).

Specifications

CAS number: 31852-29-6

Working concentration: 30 ng- 10 μg/ml

Endotoxin level:

Solubility: 5 mg/ml in physiological water (NaCl 0.9%) heated for 10 minutes at 65 - 70°C

Contents

• Poly(I:C) is provided lyophilized and is available in two quantities:

- 10 mg: (catalog # tlrl-pic)
- 50 mg: (catalog # tlrl-pic-5)

• Sterile endotoxin-free physiological water (NaCl 0.9%), 10 ml is provided with catalog #tlrl-pic and 2 x 25 ml with catalog #tlrl-pic-5

Poly(I:C) is shipped at room temperature and should be stored at 4°C. Upon resuspension, prepare aliquots of Poly(I:C) and store at -20°C for long term storage. Store at 4°C for short term storage. Lyophilized product is stable 1 year at 4°C when properly stored. Resuspended product is stable 1 month at 4°C and 1 year at -20°C. Avoid repeated freeze-thaw cycles.

Description

Polyinosinic-polycytidylic acid (poly(I:C)) is a synthetic analog of double stranded RNA (dsRNA), a molecular pattern associated with viral infection. Both natural and synthetic dsRNAs are known to induce type I interferons (IFN) and other cytokines production. Poly(I:C) is recognized by Toll-like receptor 3 (TLR3) [1, 2]. Upon poly(I:C) recognition, TLR3 activates the transcription factor interferon regulatory factor 3 (IRF3), through the adapter protein Toll-IL-1 receptor (TIR) domain-containing adapter inducing IFN-β (TRIF, also known as TICAM-1) [3]. Activation of IRF3 leads to the production of type I IFNs, especially IFN-β. A second pathway involves the recruitment of TNF receptor-associated factor 6 (TRAF6) or receptor interacting protein 1 (RIP1), with the subsequent activation of the transcription factors NF-κB and AP-1 [4]. Activation of this pathway triggers the production of inflammatory cytokines and chemokines such as TNF-α, IL-6 and CXCL10. Poly(I:C) is also recognized by the cytosolic RNA helicases retinoic acid-inducible protein I (RIG-I) and melanoma differentiation-associate gene 5 (MDA-5) [5].

1. Alexopoulou L. et al., 2001. Recognition of double-stranded RNA and activation of NFkappaB by Toll-like receptor 3. Nature, 413(6857):732-8.
2. Matsumoto M. et al., 2002. Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double- stranded RNA-mediated signaling. BBRC, 293(5):1364-9.
3. Yamamoto M. et al., 2003. Role of Adaptor TRIF in the MyD88-Independent Toll-Like Receptor Signaling Pathway. Science 301: 640.
4. Kawai T & Akira S., 2008. Tolllikereceptor and RIG-I-like receptor signaling. Ann N Y Acad Sci. 1143:1-20. Review.
5. Kato H. et al., 2006. Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses. Nature. 441(7089):101-5.

Citations

Recent articles using InvivoGen Poly(I:C) HMW

ORDERING

Poly(I:C) (HMW)

Description Long synthetic analog of dsRNA
Cat. Codetlrl-pic
Unit Size10 mg
Price For price or distributor address,
please select your country

Poly(I:C) (HMW)

Description Long synthetic analog of dsRNA
Cat. Codetlrl-pic-5
Unit Size50 mg
Price For price or distributor address,
please select your country
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
Copyrights © 2011-2016 InvivoGen. All Rights Reserved. Reproduction of any materials from this site is strictly forbidden without permission for commercial use. Nonprofit use for non-commercial research and educational purposes is permitted, citation should include the URL "www.invivogen.com".