Design hairpin insert

This tool generates the sequence of two complementary oligonucleotides necessary to create the hairpin insert for psiRNA cloning vectors.


Instructions

1. Enter the siRNA hairpin name for reference (optional)
2. Enter the sense siRNA target sequence. (Spaces in the sequence will be ignored)
3. Enter the desired loop sequence. (The default sequence is the one used by InvivoGen for psiRNA vectors).
4. Select your psiRNA cloning vector.

Our vectors offer two cloning options : Bbs I / Bbs I and Acc 65I / Hind III.




5' 3'


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psiRNA™ vectors

InvivoGen provides a plasmid-based system developed to knockdown efficiently the expression of a wide variety of mammalian genes.

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pCpGfree-siRNA vectors

pCpGfree-siRNA is a plasmid that combines the backbone of the pCpGfree plasmids with the siRNA expression cassette of the psiRNA plasmids.

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Ready-made psiRNA

Ready-made psiRNA is a family of plasmids expressing a growing list of shRNAs which functionality has been described in the literature or validated in house.

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Selective Antibiotics

Our state-of-the-art facilities allow us to produce large quantities of high quality, endotoxin-free antibiotics with purity levels exceeding 95%.

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The heart of InvivoGen's culture and commitment is to provide life scientists with quality and reliable reagents. Our strength in innovation comes from our expert scientific team that fosters the discovery and design of new tools and services to encourage cutting-edge bioresearch. As specialists in innate immunology, InvivoGen is a global leader of resources for the next breakthroughs in the field.

InvivoGen provides a set of cutting-edge tools to knockdown efficiently the expression of mammalian genes. These tools include the siRNA wizard software, shRNA cloning vectors and vectors expressing validated shRNAs, and systems for the prevalidation of siRNA/shRNAs or the detection of interferon response.

For more information, please visit our website