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THP1-XBlue™-MD2-CD14 Cells

THP1 -XBlue™-MD2-CD14 cells Unit size Cat. code Docs Qty Price
NF-kB/AP-1 Reporter MD2 CD14 co-transfected Monocytes
3-7 x 10e6 cells
thpx-mdcdsp
+-
$1,142.00

June, 18th 2018 - CONTENTS UPDATE NOTIFICATION
Please note that, for your convenience, these cells are now provided with QUANTI-Blue™ Solution.

NF-kB/AP-1- Reporter Monocytes stably transfected with MD2/CD14 genes

THP1-XBlue™-MD2-CD14 cells derive from the THP1-XBlue™ cell line. THP1-XBlue™-MD2-CD14 cells stably express the human MD2 and CD14 genes. MD2 is an accessory molecule essential for LPS-induced TLR4 response. CD14, a macrophage-specific differentiation antigen, interacts with several TLRs, including TLR4 and TLR2. Overexpression of CD14 was found to increase the response to the majority of TLR ligands.

THP1-XBlue™-MD2-CD14 cells express an NF-κB/AP-1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene. TLR stimulation in THP1-XBlue™-MD2-CD14 cells induces signaling cascades leading to the activation of NF-κB and AP-1 and the subsequent production of SEAP. The reporter protein is easily detectable and measurable when using QUANTI-Blue™, a medium that turns purple/blue in the presence of SEAP.

 

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Specifications

Antibiotic resistance: G418, Zeocin™.

Growth medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml).

Guaranteed mycoplasma-free.

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Contents

  • 1 vial containing 3-7 x 106 cells
  • 1 ml of G418 (100 mg/ml)
  • 1 ml of Zeocin™ (100 mg/ml)
  • 1 ml of Normocin™ (50 mg/ml)
  • 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
  • 100 μg of LPS-EK (E. coli K12 lipopolysaccharide, positive control for TLR4 activity)

Shipped on dry ice

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