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THP1-XBlue™ Cells

THP1-XBlue™ cells Unit size Cat. code Docs Qty Price
NF-kB/AP-1 Reporter Monocytes
3-7 x 10e6 cells
thpx-sp
+-
$1,035.00

June, 18th 2018 - CONTENTS UPDATE NOTIFICATION
Please note that, for your convenience, these cells are now provided with QUANTI-Blue™ Solution.

NF-kB/AP-1- Reporter Monocytes

THP1-XBlue™ cells derive from the human monocytic THP-1 cell line. THP1-XBlue™ cells stably express an NF-κB- and AP-1-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene.

Upon TLR stimulation, THP1-XBlue™ cells induce the activation of NF-κB and AP-1 and subsequently the secretion of SEAP. The reporter protein is easily detectable and measurable when using QUANTI-Blue™, a medium that turns purple/blue in the presence of SEAP.

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Specifications

Antibiotic resistance: Zeocin™

Growth medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml)

Guaranteed mycoplasma-free

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Contents

  •  1 vial containing 3-7 x 106 cells
  •  1 ml of Zeocin™ (100 mg/ml)
  •  1 ml Normocin™ (50 mg/ml)
  •  1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
  •  109 cells HKLM (Heat Killed Listeria monocytogenes; positive control of TLR2 activity)

Shipped on dry ice

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Citations

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