|THP1 -XBlue™-CD14 cells||Unit size||Cat. code||Docs||Qty||Price|
NF-kB/AP-1 Reporter CD14 transfected Monocytes
3-7 x 10e6 cells
NF-kB/AP-1- Reporter Monocytes
THP1-XBlue™-CD14 cells derive from the THP1-XBlue™ cell line. THP1-XBlue™-CD14 cells stably express the human CD14 gene, encoding a macrophage-specific differentiation antigen. CD14 interacts with several TLRs in the induction of the signaling cascade upon TLR stimulation and its overexpression was found to increase the reponse to the majority of TLR ligands.
THP1-XBlue™-CD14 cells express an NF-κB/AP-1-inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene. TLR stimulation in THP1-XBlue™-CD14 cells induces signaling cascades leading to the activation of NF-κB and AP-1 and the subsequent production of SEAP. The reporter protein is easily detectable and measurable when using QUANTI-Blue™, a medium that turns purple/blue in the presence of SEAP.
Growth medium: RPMI 1640, 2 mM L-glutamine, 25 mM HEPES, 10% heat-inactivated fetal bovine serum, 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml)
Guaranteed mycoplasma-freeBack to the top
- 1 vial containing 3-7 x 106 cells
- 100 μl G418 (100 mg/ml)
- 100 μl Zeocin™ (100 mg/ml)
- 1 ml Normocin™ (50 mg/ml)
- 1 pouch of QUANTI-Blue™ (100 ml)
- 100 μg LPS-EK (E. coli K12 lipopolysaccharide, positive control for TLR4 activity)
Shipped on dry iceBack to the top