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Mincle Agonist - Synthetic cord factor analog formulated with HS15
Synthetic cord factor analog
Trehalose-6,6-dibehenate formulated with Kolliphor® HS 15
Trehalose-6,6-dibehenate (TDB) is a non-toxic synthetic analogue of trehalose-6,6-dimycolate (TDM, also known as cord factor), which is the most studied immunostimulatory component of Mycobacterium tuberculosis.
TDB is an effective adjuvant for Th1/Th17 vaccination. TDB was found to rely on Mincle, Syk and Card9 for its adjuvant activity . TDB is a poorly soluble compound and thus was formulated with Kolliphor® HS 15, a potent low-toxicity non-ionic solubilizer, to generate TDB-HS15, which is particularly suitable for in vivo studies.
TDB-HS15 is a strong activator of NF-kB in a Mincle-dependent manner.
Kolliphor® HS 15 (former tradename Solutol® HS 15) is a registered trademark of BASF.
1. Schoenen H, et al., 2010. Cutting edge: Mincle is essential for recognition and adjuvanticity of the mycobacterial cord factor and its synthetic analog trehalose-dibehenate. J Immunol. ; 184(6):2756-60.Back to the top
Specificity: Mincle agonist
CAS number: 66758-35-8
Molecular weight: 987.43
Working concentration: 0.3 - 100 µg/mlBack to the top
- 2 x 1 mg Trehalose-6,6-dibehenate (TDB) formulated with Kolliphor® HS 15
Responses to M. tuberculosis-derived ligands.
RAW-Blue™ cells, and HEK-Blue™ hMincle, HEK-Blue™ TLR2 or HEK-Blue™ TLR4 cells, which express the human mincle, TLR2 or TLR4 gene, respectively, were stimulated with 10 µg/ml of M. tuberculosis-derived ligands. After 24h incubation, NF-κB activation was assessed by measuring the levels of SEAP using the QUANTI-Blue™ assay.