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TDB-HS15

TDB-HS15 Unit size Cat. code Docs Qty Price
Mincle Agonist - Synthetic cord factor analog formulated with HS15
2 mg
tlrl-stdb
+-
$219.00

Synthetic cord factor analog
Trehalose-6,6-dibehenate formulated with Kolliphor® HS 15

Trehalose-6,6-dibehenate (TDB) is a non-toxic synthetic analogue of trehalose-6,6-dimycolate (TDM, also known as cord factor), which is the most studied immunostimulatory component of Mycobacterium tuberculosis.

TDB is an effective adjuvant for Th1/Th17 vaccination. TDB was found to rely on Mincle, Syk and Card9 for its adjuvant activity [1]. TDB is a poorly soluble compound and thus was formulated with Kolliphor® HS 15, a potent low-toxicity non-ionic solubilizer, to generate TDB-HS15, which is particularly suitable for in vivo studies.

TDB-HS15 is a strong activator of NF-kB in a Mincle-dependent manner. TDB-HS15 is available in a standard grade and VacciGrade™.

Kolliphor® HS 15 (former tradename Solutol® HS 15) is a registered trademark of BASF.

1. Schoenen H, et al., 2010. Cutting edge: Mincle is essential for recognition and adjuvanticity of the mycobacterial cord factor and its synthetic analog trehalose-dibehenate. J Immunol. ;184(6):2756-60.

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Specifications

Specificity: Mincle agonist
CAS number: 66758-35-8
Formula: C56H106O13
Molecular weight: 987.43
Working concentration: 0.3 - 100 µg/ml

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Contents

- 2 x 1 mg Trehalose-6,6-dibehenate (TDB) formulated with Kolliphor® HS 15

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Details

Responses to M. tuberculosis-derived ligands.

Responses to M. tuberculosis-derived ligands.
RAW-Blue™ cells, and HEK-Blue™ hMincle, HEK-Blue™ TLR2 or HEK-Blue™ TLR4 cells, which express the human mincle, TLR2 or TLR4 gene, respectively, were stimulated with 10 µg/ml of M. tuberculosis-derived ligands. After 24h incubation, NF-κB activation was assessed by measuring the levels of SEAP using the QUANTI-Blue™ assay.

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