Streptavidin-Lucia
| Streptavidin-Lucia | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
Bioluminescent conjugate of Streptavidin |
2 x 500 µl |
rep-svlc |
Bioluminescent conjugate of Streptavidin
The common techniques of ELISA and Western Blot analysis widely use the Streptavidin-Biotin system for signal amplification. Streptavidin-Lucia features the Lucia luciferase. Streptavidin-Lucia has been optimized for ELISA and can be adapted for use in a variety of assays requiring the detection of biotin and biotinylated proteins.
Streptavidin-Lucia consists of a streptavidin peptide fused to the Lucia luciferase, a small secreted coelenterazine-utilizing luciferase with strong and stable bioluminescence properties.
Streptavidin-Lucia uses the advantages of two systems, streptavidin for strength of binding to biotin labeled proteins, together with an optimized luciferase with a strong and stable bioluminescence for high sensitive detection with low background.
Thus, this product is used for the detection of streptavidin-biotin interactions with high sensitivity and accuracy.
The unique long-lasting bioluminescence property of Lucia luciferase allows for ease in reading measurements. After addition of the QUANTI-Luc™ assay reagent to each well of a 96-well plate, results can be obtained in less than a minute using a luminometer without injectors.
Specifications
Highly sensitive : Detect low levels of target with low background
Accurate : Tight linear correlation over several logs
Convenient : Reveal using one-step QUANTI-Luc™ detection reagent
Rapid : Measure 96 samples in a microplate in less than a minute
Streptavidin-Lucia has been optimized for ELISA and can be adapted for use in a variety of assays requiring the detection of biotin and biotinylated proteins.
ELISA: 1:1000 dilution of stock solution
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- 2 tubes of lyophilized Streptavidin-Lucia
Once resuspended, each tube contains 500 µl of Streptavidin-Lucia
Streptavidin-Lucia is shipped at room temperature
Stored at -20°C.
Lyophilized product is stable 1 year when properly stored.
Description
PRODUCT DESCRIPTION
The synthetic Lucia luciferase gene was fused to the Streptomyces avidinii streptavidin gene using a linker to generate a recombinant Streptavidin-Lucia protein.
The Streptavidin-Lucia protein has a molecular mass of ~37 kDa and was produced in CHO cells.
Streptavidin-Lucia combines the advantages of two systems:
streptavidin, for strength of binding to biotin-labeled proteins, and Lucia luciferase, a small secreted coelenterazine-utilizing luciferase with strong and stable bioluminescence properties for sensitive detection with low background.
This product can be used for the detection of streptavidin-biotin interactions with high sensitivity, accuracy and conveniency when paired with QUANTI-Luc™, a coelenterazine-utilizing luciferase assay reagent.
The unique long-lasting bioluminescence property of Lucia luciferase allows for ease in reading measurements. After addition of the QUANTI-Luc reagent to each well of a 96-well plate, results can be obtained in less than a minute using a luminometer without injectors.
Back to the topDetails
Typical data obtained using Strepavidin-Lucia for the detection of cytokines with commercially available ELISA. Graph shows detection of hIL1β, hIL8 and hIL6.
Streptavidin-Lucia was detected using QUANTI-Luc™. Linear regression indicates detection range over at least 3 logs.