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Streptavidin-Lucia

Streptavidin-Lucia Unit size Cat. code Docs Qty Price
Bioluminescent conjugate of Streptavidin
2 x 500 µl
rep-svlc
+-
$146.00

Bioluminescent conjugate of Streptavidin

The common techniques of ELISA and Western Blot analysis widely use the Streptavidin-Biotin system for signal amplification. Streptavidin-Lucia features the Lucia luciferase. Streptavidin-Lucia has been optimized for ELISA and can be adapted for use in a variety of assays requiring the detection of biotin and biotinylated proteins.

Streptavidin-Lucia consists of a streptavidin peptide fused to the Lucia luciferase, a small secreted coelenterazine-utilizing luciferase with strong and stable bioluminescence properties.
Streptavidin-Lucia uses the advantages of two systems, streptavidin for strength of binding to biotin labeled proteins, together with an optimized luciferase with a strong and stable bioluminescence for high sensitive detection with low background.
Thus, this product is used for the detection of streptavidin-biotin interactions with high sensitivity and accuracy.

The unique long-lasting bioluminescence property of Lucia luciferase allows for ease in reading measurements. After addition of the QUANTI-Luc™ assay reagent to each well of a 96-well plate, results can be obtained in less than a minute using a luminometer without injectors.
 

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Specifications

Highly sensitive : Detect low levels of target with low background

Accurate : Tight linear correlation over several logs

Convenient : Reveal using one-step QUANTI-Luc™ detection reagent

Rapid : Measure 96 samples in a microplate in less than a minute

Streptavidin-Lucia has been optimized for ELISA and can be adapted for use in a variety of assays requiring the detection of biotin and biotinylated proteins.

ELISA: 1:1000 dilution of stock solution

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Contents

  • 2 tubes of lyophilized Streptavidin-Lucia

Once resuspended, each tube contains 500 µl of Streptavidin-Lucia

room temperature Streptavidin-Lucia is shipped at room temperature

store Stored at -20°C.

stable Lyophilized product is stable 1 year when properly stored.

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Description

PRODUCT DESCRIPTION

The synthetic Lucia luciferase gene was fused to the Streptomyces avidinii streptavidin gene using a linker to generate a recombinant Streptavidin-Lucia protein.

The Streptavidin-Lucia protein has a molecular mass of ~37 kDa and was produced in CHO cells.

Streptavidin-Lucia combines the advantages of two systems:
streptavidin, for strength of binding to biotin-labeled proteins, and Lucia luciferase, a small secreted coelenterazine-utilizing luciferase with strong and stable bioluminescence properties for sensitive detection with low background.

This product can be used for the detection of streptavidin-biotin interactions with high sensitivity, accuracy and conveniency when paired with QUANTI-Luc™, a coelenterazine-utilizing luciferase assay reagent.

The unique long-lasting bioluminescence property of Lucia luciferase allows for ease in reading measurements. After addition of the QUANTI-Luc reagent to each well of a 96-well plate, results can be obtained in less than a minute using a luminometer without injectors.

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Details

Typical data obtained using Strepavidin-Lucia for the detection of cytokines with commercially available ELISA.

Typical data obtained using Strepavidin-Lucia for the detection of cytokines with commercially available ELISA. Graph shows detection of hIL1β, hIL8 and hIL6.
Streptavidin-Lucia was detected using QUANTI-Luc™. Linear regression indicates detection range over at least 3 logs.

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