J774.1 cells express a variety of pattern recognition receptors (PRRs), including Toll-like receptors (TLRs) [1, 2], C-type lectin receptors (CLRs) [2,3], RIG-I-like receptors (RLRs) [4].

Upon recognition of their cognate PAMPs, these receptors induce signaling pathways leading to the activation of the
transcription factors NF-kB and/or IRF3/7. Stimulation of J774-Dual™ cells with the following PAMPs, Pam3CSK4 (TLR1/2), lipopolisaccharide (TLR4), CpG ODNs (TLR9), L18-MDP (NOD2) and TDB (Mincle), leads to the activation of NF-kB.
Stimulation with RLR ligands, such as transfected poly(I:C) or 5’ppp-dsRNA, or the STING agonist, 2’3’-cGAMP, triggers the IRF pathway.

J774-Dual™ cells are resistant to the selectable markers blasticidin and Zeocin®.

1. Jin M. et al., 2011. Effects of chondroitin sulfate and its oligosaccharides on toll-like receptor-mediated IL-6 secretion by macrophage-like J774.1 cells. Biosci Biotechnol Biochem. 75(7):1283-9.
2. Kushida T. et al., 2011. Enhancement of Dectin-2 gene expression by lignin-carbohydrate complex from Lentinus edodes mycelia extract (LEM) in a mouse macrophage-like cell line. Anticancer Res. 31(4):1241-8.
3. Takeda Y. et al., 2007. Ternary complex consisting of DNA, polycation, and a natural polysaccharide of schizophyllan to induce cellular uptake by antigen presenting cells. Biomacromolecules. 8(4):1178-86.
4. Wilden H. et al., 2009. Expression of RIG-I, IRF3, IFN-beta and IRF7 determines resistance or susceptibility of cells to infection by Newcastle Disease Virus. Int J Oncol. 34(4):971-82.

Clear allRefined by

Products1

1 Search results: j774d-nfis