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Human Type I IFNs Reporter Cells

Signaling pathway in HEK-Blue™ IFN-α/β cells

InvivoGen also offers:

• Recombinant human IFN-α2b
• Anti-hIFNAR (Anifrolumab)
• Other IFN-α subtypes upon request

HEK-Blue™ IFN-α/β cells were engineered from the human embryonic kidney HEK 293 cell line to detect bioactive human type I interferons (e.g. IFN-α, IFN-β) by monitoring the activation of the JAK/ISGF3 (STAT1/STAT2/IRF9 complex) pathway. In addition, these cells can be used for screening antibodies or small molecule inhibitors targeting the IFN-α/β pathway.
IFN-α and IFN-β are important anti-viral cytokines that also have anti-proliferative and immunomodulatory functions [1, 2].

More details

Cell line description

HEK-Blue™ IFN-α/β cells were generated by stable transfection with the genes encoding human STAT2 and IRF9 to obtain a fully active type I IFN signaling pathway. The other genes of the pathway (IFNAR1, IFNAR2, JAK1, TyK2, and STAT1) are naturally expressed by these cells. HEK-Blue™ IFN-α/β cells were also stably transfected with the secreted embryonic alkaline phosphatase (SEAP) reporter under the control of the ISG54 promoter. This promoter comprises IFN-stimulated response elements (ISRE) that are recognized by the ISGF3 complex. The binding of IFN-α or IFN-β to their receptor triggers a signaling cascade leading to the activation of ISGF3 and the subsequent production of SEAP. This can be readily assessed in the supernatant using QUANTI-Blue™ Solution, a SEAP detection reagent.

HEK-Blue™ IFN-α/β cells detect human (h) IFN-α and hIFN-β. At high concentrations, they might respond to mouse (m) IFN-α, but not mIFN-β (see figures). Of note, HEK-Blue™ IFN-α/β cells do not respond to human type II and type III IFNs (IFN-γ/λ) (see figures).

Key Features

Fully functional human IFN-α/β signaling pathway
Readily assessable ISGF3-inducible SEAP reporter activity
Strong response to human (h) IFN-α and hIFN-β
Poor to no response to murine (m) IFN-α and mIFN-β
No response to IFN-γ and IFN-λ

Applications

Detection of human IFN-α and IFN-β
Quantification of IFN-α/β activity levels in biological samples, such as plasma or serum [3]
Screening of anti-IFN-α/β and anti-IFNAR antibodies
Screening of small molecule inhibitors of the IFN-α/β pathway

Note: A new clone is provided with an improved Type I IFN response. The cat code has been changed accordingly (hkb-ifnabv2).

References:

1. Schreiber G. 2017. The molecular basis for differential type I interferon signaling. J. Biol. Chem. 292:7285-94.
2. McNab F. et al., 2015. Type I interferons in infectious disease. Nat Rev Immunol. 15(2):87-103.
3. Gómez-Bañuelos E, et al., 2024. Uncoupling interferons and the interferon signature explain clinical and transcriptional subsets in SLE. medRxiv. 2023.08.28.23294734

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