SEAP Reporter Assay Kit
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Reagent kit to determine level of SEAP
Reagents kit to determine the levels of SEAP in the culture medium
The SEAP Reporter Assay Kit provides a rapid and convenient method to determine the levels of SEAP (Secreted embryonic alkaline phosphatase) in the culture medium of transfected cells expressing the seap gene.
SEAP catalyzes the hydrolysis of p-Nitrophenyl phosphate producing a yellow end product that can be read spectrophotometrically at 405 nm.Back to the top
Short hands-on-time: less than 1 hour
Obtention of results: 10 to 40 minutes
Sensitivity: as little as 10-10 g/ml of SEAP
Concentration range: 1 ng - 1 µg/mlBack to the top
The SEAP Reporter Assay Kit contains all reagents necessary to measure SEAP activity in 150 samples.
- 10X Dilution Buffer (2 x 1 ml)
- 5X Assay Buffer (4 x 1 ml)
- 100 mM L-Homoarginine (4 x 1 ml)
- Tablets of SEAP substrate (20 tablets)
The SEAP Reporter Assay Kit is shipped at room temperature.Back to the top