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SEAP Reporter Assay Kit

SEAP Reporter Assay Kit Unit size Cat. code Docs Qty Price
Reagent kit to determine level of SEAP
1 kit
rep-sap
+-
$174.00

December 17th2018 - CONTENTS UPDATE NOTIFICATION
We now provide the dilution buffer at 10X concentration (2 x 1 ml).

Reagents kit to determine the levels of SEAP in the culture medium

The SEAP Reporter Assay Kit provides a rapid and convenient method to determine the levels of SEAP (Secreted embryonic alkaline phosphatase) in the culture medium of transfected cells expressing the seap gene.

SEAP catalyzes the hydrolysis of p-Nitrophenyl phosphate producing a yellow end product that can be read spectrophotometrically at 405 nm.

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Specifications

Short hands-on-time:  less than 1 hour

Obtention of results: 10 to 40 minutes

Sensitivity: as little as 10-10 g/ml of SEAP

Concentration range: 1 ng - 1 µg/ml

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Contents

The SEAP Reporter Assay Kit contains all reagents necessary to measure SEAP activity in 150 samples.

 

  • 10X Dilution Buffer (2 x 1 ml)
  • 5X Assay Buffer (4 x 1 ml)
  • 100 mM L-Homoarginine (4 x 1 ml)
  • Tablets of SEAP substrate (20 tablets)

 

The SEAP Reporter Assay Kit is shipped at room temperature.

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Citations

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