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Dectin-1 Agonist - Beta-glucan from Schizophyllum commune
Beta-glucan from Schizophyllum commune - β(1➝3, 1➝6)-glucan
Schizophyllan (SPG) is a gel-forming β-glucan from the fungus Schizophyllum commune.
SPG is a high molecular weight (450 kDa) (1-3)-β-D-glucan that has a 1,6-β-monoglucosyl branch in every three 1,3-β-glucosyl residues on the main chain.
SPG binds to Dectin-1  and triggers a signaling cascade leading to NF-κB activation. Detection of β-glucans by Dectin-1 receptor leads to the CARD9-dependent activation of NF-κB and MAP kinases .
1. Adachi Y. et al., 2004. Characterization of beta-glucan recognition site on C-type lectin, dectin 1. Infect Immun. 72(7):4159-71.
2. Goodridge HS. et al., 2009. Beta-glucan recognition by the innate immune system. Immunol Rev. 230(1):38-50.
Specificity: Dectin-1 ligand
CAS number: 9050-67-3
Partial structure: β(1➝3, 1➝6)-glucan
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- 100 mg schizophyllan provided as a powder
Schizophyllan is shipped at room temperature.
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Responses to β-glucans.
RAW-Blue™ cells and HEK-Blue™ mDectin-1 cells, which express the mouse dectin-1a gene, were stimulated with 100 µg/ml of various β-glucans.
After 24h incubation, NF-κB activation was assessed by measuring the levels of SEAP (secreted embryonic alkaline phosphatase) using the QUANTI-Blue™ assay.
Stimulatory activity of Dectin-1 ligands:
HEK-Blue™ hDectin-1a and HEK-Blue™ hDectin-1b cells were stimulated with 1 µg/ml or 10 µg/ml Dectin-1 ligands, respectively, and 10 µg/ml TDB.
After 24h incubation, Dectin-1-induced NF-κB activation was assessed by measuring the levels of SEAP using the QUANTI-Blue™ assay.