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Viral RdRp inhibitor
Remdesivir, also known as Veklury or GS-5734, is a 1’-cyano-substituted adenosine nucleotide analog prodrug that shows broad-spectrum antiviral activity. It was originally developed as an anti-viral against Ebola virus , but since has been shown to attenuate viral loads of a number of RNA viruses including respiratory syncytial virus (RSV) and β-coronaviruses such as SARS-CoV, MERS-CoV, and the causative agent of COVID-19, SARS-CoV-2 [2-3].
Remdesivir specifically inhibits the activity of the viral RNA-dependent RNA-polymerase (RdRp), essential in viral replication [3-5]. Upon entry into the cell, Remdesivir is rapidly metabolized into a nucleoside monophosphate (GS-441542 MP), which is then further processed into an active triphosphate form (GS-441524). GS-441524 is an adenosine triphosphate (ATP) analog and is thus, able to be used as a substrate by viral RdRp. GS-441524 outcompetes ATP for incorporation into the newly synthesized RNA strand, ultimately causing premature termination of the RNA product. However, unlike classic chain-terminators, the incorporation of GS-441524 causes delayed chain-termination downstream of this site [3, 4]. Importantly, it has been established that GS-441524 evades proofreading by the viral exoribonuclease (ExoN) .
Remdesivir has been shown to have no significant inhibitory activity on human RNA Pol II and mitochondrial RNA polymerase (h-mtRNAP) . Recently, Remdesivir has been shown to exhibit protective effects in vitro against non-alcoholic fatty liver disease (NAFLD), by inhibiting pro‑inflammatory signaling mediated by STING .
Key features of Remdesivir:
- Metabolized intracellularly into an active adenosine triphosphate analog (GS-441524)
- Potent inhibitor of viral RdRp function
- Highly pure (≥ 95%) and the absence of bacterial contamination has been confirmed in cellular assays.
InvivoGen’s products are for research use only, and not for clinical or veterinary use.
Read our reviews on COVID-19 and learn more about SARS-CoV-2 infection cycle, immune responses, and potential therapeutics.
1. Warren, T.K. et al. 2016. Therapeutic efficacy of the small molecule GS-5734 against Ebola virus in rhesus monkeys. Nature 531, 381-385.
2. Gordon, C.J. et al. 2020. The antiviral compound remdesivir potently inhibits RNA-dependent RNA polymerase from Middle East respiratory syndrome coronavirus. J Biol Chem
3. Gordon, C.J. et al. 2020. Remdesivir is a direct-acting antiviral that inhibits RNA-dependent RNA polymerase from severe acute respiratory syndrome coronavirus 2 with high potency. J Biol Chem 295, 6785-6797.
4. Tchesnokov, E.P. et al. 2019. Mechanism of Inhibition of Ebola Virus RNA-Dependent RNA Polymerase by Remdesivir. Viruses 11
5. Agostini, M.L. et al. 2018. Coronavirus Susceptibility to the Antiviral Remdesivir (GS-5734) Is Mediated by the Viral Polymerase and the Proofreading Exoribonuclease. mBio 9
6. Li, Y.N. & Su, Y. 2020. Remdesivir attenuates high fat diet (HFD)-induced NAFLD by regulating hepatocyte dyslipidemia and inflammation via the suppression of STING. Biochem Biophys Res Commun 526, 381-388.
CAS number: 1809249-37-3
Molecular weight: 602.58 g/mol
Solubility: 100 mg/ml (165.95 mM) in DMSO and 25 mg/ml (41.48 mM) in ethanol
- 0.01 - 1 µM (viral RdRp inhibition in published cell culture assays) 
Note: Cellular cytotoxicity is observed at concentrations greater than 10 µM (CC50 > 10µM) 
- Purity: ≥95% (UHPLC)
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ hTLR2 and HEK-Blue™ hTLR4 cells.
- 1 mg Remdesivir (provided as a dried powder)
This product is shipped at room temperature.
Upon receipt, store at -20 °C. Upon resuspension of Remdesivir in DMSO, prepare aliquots and store at -20 °C.
Resuspended product is stable for at least 3 months when properly stored.
Avoid repeated freeze-thaw cycles.Back to the top
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