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psiRNA-h7SK

psiRNA-h7SK G1 GFPzeo Unit size Cat. code Docs Qty Price
Kit to create siRNAs - GFPzeo
1 kit
ksirna4-gz21
+-
$400.00
psiRNA-h7SK G1 Blasti Unit size Cat. code Docs Qty Price
Kit to create siRNAs - Blasti
1 kit
ksirna3-b21
+-
$359.00
discontinued
psiRNA-h7SK G1 Hygro Unit size Cat. code Docs Qty Price
Kit to create siRNAs - Hygro
1 kit
ksirna3-h21
+-
$359.00
discontinued
psiRNA-h7SK G1 Neo Unit size Cat. code Docs Qty Price
Kit to create siRNAs - Neo
1 kit
ksirna3-n21
+-
$359.00
discontinued
psiRNA-h7SK G1 Zeo Unit size Cat. code Docs Qty Price
Kit to create siRNAs - Zeo
1 kit
ksirna3-z21
+-
$359.00
discontinued

7SK-based expression of shRNAs

psiRNA-h7SK is a family of expression vectors designed to generate shRNAs from the human 7SK RNA polymerase III promoter.

7SK is an abundant and evolutionarily conserved small nuclear RNA transcribed by RNA polymerase III [1]. The human 7SK promoter is ideal for the production of shRNAs as it can generate high amounts of shRNAs [1, 2].

It has been shown to provide better silencing efficiencies of various target genes than the human H1 and U6 promoters [3].

Furthermore, the human 7SK promoter can be used in combination with other RNA pol III promoters for multiple shRNA expression strategies [4].

psiRNA-h7SK has been successfully used to achieve gene silencing in vitro and in vivo.

 

References:

1. Czauderna F. et al., 2003. Inducible shRNA expression for application in a prostate cancer mouse model. Nucleic Acids Res. 31(21):e127.
2. Koper-Emde D. et al., 2004. RNA interference by small hairpin RNAs synthesised under control of the human 7S K RNA promoter. Biol Chem. 385(9):791-4.
3. Gao Z. et al., 2017.
Mutation of nucleotides around the +1 position of type 3 polymerase III promoters: The effect on transcriptional activity and start site usage. Transcription. 8(5):275-87. 
4. Mazzanti CM. et al., 2004. Early genetic mechanisms underlying the inhibitory effects of endostatin and fumagillin on human endothelial cells. Genome Res. 14(8):1585-93.

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Specifications

Two cloning options: Acc 65I / Hind III or Bbs I / Bbs I

Allows white and blue selection

Provided with the psiRNA™ tools:

  • 1 disk of lyophilized GT116 E. coli bacteria
  • Sequencing primers (OL559-OL408)
  • Selection antibiotic
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Contents

Each psiRNA-h7SK plasmid is provided with the following components:

  • 20 μg of the psiRNA-h7SK plasmid
  • 20 μg of control plasmid 1 (targets the EGFP or LacZ reporter genes)
  • 20 μg of control plasmid 2 (targets the luciferase GL3 reporter gene)
  • 1 disk of lyophilized GT116 E. coli bacteria (GT116 genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZM15 ΔlacX74 recA1 endA1 ΔsbcC-sbcD)
  • 10 μg of OL559 primer (forward) and 10 μg of OL408 primer (reverse)
  • 1 ml of Zeocin
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Details

psiRNA-h7SK backbone

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Citations

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