|pSELECT-hHMGB1-Lucia||Unit size||Cat. code||Docs||Qty||Price|
hHMGB1::Lucia fusion gene
|pSELECT-mHMGB1-Lucia||Unit size||Cat. code||Docs||Qty||Price|
mHMGB1::Lucia fusion gene
Expression-ready ORF Clones
pSELECT-HMGB1-Lucia are mammalian vectors designed for expression of the human or murine HMGB1::Lucia fusion genes. These plasmids are selectable in bacteria and mammalian cells with Zeocin™.
pSELECT-HMGB1::Lucia plasmids contain two expression cassettes.
- The first one delivers strong and constitutive expression of HMGB1::Lucia.
- The second one drives the zeocin-resistance gene (Sh ble) that allows the selection of stable transfectants (and selection in bacteria).
Each cassette terminates with a strong polyadenylation signal (polyA) thus preventing any transcription interference. In order to obtain enough copies for HMGB1::Lucia expression in mammalian cells, we recommend to use this plasmid for viral gene transfer.
Expression of HMGB1::Lucia in cells equipped for inflammasome mediated-pyroptosis and/or necroptosis allows monitoring of these forms of necrotic cell death.Back to the top
Gene: HMGB1::Lucia fusion gene
Promoter: hEF1-HTLVBack to the top
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20 ̊C.
Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.
Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.
Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.
Avoid repeated freeze-thaw cycles.Back to the top