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HMGB1-Lucia

pSELECT-hHMGB1-Lucia Unit size Cat. code Docs Qty Price
hHMGB1::Lucia fusion gene
20 µg
psetz-hhmgbluc
+-
$448.00
pSELECT-mHMGB1-Lucia Unit size Cat. code Docs Qty Price
mHMGB1::Lucia fusion gene
20 µg
psetz-mhmgbluc
+-
$448.00

Expression-ready ORF Clones

 

pSELECT-HMGB1-Lucia are mammalian vectors designed for expression of the human or murine HMGB1::Lucia fusion genes. These plasmids are selectable in bacteria and mammalian cells with Zeocin™.

pSELECT-HMGB1::Lucia plasmids contain two expression cassettes.

  • The first one delivers strong and constitutive expression of HMGB1::Lucia.
  • The second one drives the zeocin-resistance gene (Sh ble) that allows the selection of stable transfectants (and selection in bacteria).

Each cassette terminates with a strong polyadenylation signal (polyA) thus preventing any transcription interference. In order to obtain enough copies for HMGB1::Lucia expression in mammalian cells, we recommend to use this plasmid for viral gene transfer.

Expression of HMGB1::Lucia in cells equipped for inflammasome mediated-pyroptosis and/or necroptosis allows monitoring of these forms of necrotic cell death.

Figures

pSELECT-zeo-hHMGB1-Lucia
pSELECT-zeo-hHMGB1-Lucia
pSELECT-zeo-mHMGB1-Lucia
pSELECT-zeo-mHMGB1-Lucia
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Specifications

Selection: Zeocin™

Gene: HMGB1::Lucia fusion gene

Promoter: hEF1-HTLV

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Contents pUNO1

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml

 

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20 ̊C.

stability Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.

Alert Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.

 

Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.

Avoid repeated freeze-thaw cycles.

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