|Poly(dT)||Unit size||Cat. code||Docs||Qty||Price|
Thymidine homopolymer ODN (17 mer)
Poly(dT), a thymidine homopolymer phosphorothioate ODN, is a modulator of human TLR7 and TLR8. In combination with an imidazoquinoline, such as R848 and CL075, it increases TLR8-mediated signaling but abolishes TLR7-mediated signaling [1, 2]. Alone poly(dT) has no significant effect on either of these TLRs. Furthermore, co-incubation of poly(dT) and an imidazoquinoline was shown to induce NF-κB activation in HEK293 cells transfected with murine TLR8- and primary TLR8-expressing mouse cells, demonstrating that murine TLR8 is functional .
1. Jurk M. et al., 2006. Modulating responsiveness of human TLR7 and 8 to small molecule ligands with T-rich phosphorothiate oligodeoxynucleotides. Eur J Immunol. 36(7):1815-26.
2. Gorden KKB. et al., 2006. Oligodeoxynucleotides Differentially Modulate Activation of TLR7 and TLR8 by Imidazoquinolines. J. Immunol. 177: 8164 - 8170.
3. Gorden KKB. et al., 2006. Cutting Edge: Activation of Murine TLR8 by a Combination of Imidazoquinoline Immune Response Modifiers and PolyT OligodeoxynucleotidesJ. Immunol., 177: 6584 - 6587.
Specificity: TLR7/8 modulator
Working Concentration: 10 μM
Endotoxin level: EndoFit™ (<0.001 EU/µg)
Solubility: 100 nmol/ml in water
• 100 nmol poly(dT), a 17-mer phosphorothioate polyT ODN
• 2 ml sterile endotoxin-free water
Poly(dT) is provided as a sterile white lyophilized powder and shipped at room temperature. Store at -20 ̊C. Resuspended poly(dT) should be aliquoted and stored at -20 ̊C for long term storage. Store at 4°C for short term storage. Lyophilized product is stable 1 year at -20°C when properly stored. Resuspended product is stable 1 month at 4°C and 6 months at -20°C.Back to the top