pFUSE-hIgG1e3-Fc1 Unit size Cat. code Docs Qty Price
20 µg
pFUSE-hIgG1e3-Fc2 Unit size Cat. code Docs Qty Price
20 µg

pFUSE-hIgG1e3-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the mutated human IgG1 heavy chain and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently.  The engineered hIgG1e3 Fc contains the following mutations: E233P/L234V/L235A/ΔG236 + A327G/A330S/P331S.

pFUSE-hIgG1e3-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted.

DISCLAIMER: pFUSE-hIgG1e3 plasmids featuring the IgG1e3 engineered Fc are sold by InvivoGen under a limited license from Cambridge Enterprise Limited (International Publication Number WO 99/58572).

Armour KL. et al., 1999. Recombinant human IgG molecules lacking Fcgamma receptor I binding and monocyte triggering activities. Eur J Immunol. 29(8):2613-24.
Shields RL. et al., 2001. High resolution mapping of the binding site on human IgG1 for Fc gamma RI, Fc gamma RII, Fc gamma RIII, and FcRn and design of IgG1 variants with improved binding to the Fc gamma R. J Biol Chem. 276(9):6591-604.

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Isotype: IgG1 human
Mutations: E233P/L234V/L235A/ΔG236 + A327G/A330S/P331S
- Reduced binding to FcγRI
- Reduced ADCC and CDC

Both plasmids are selectable with Zeocin™.

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• 20 μg of lyophilized DNA
• 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)

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