Pam3CSK4 Rhodamine

Rhodamine-labeled synthetic triacylated lipopeptide

Pam3CSK4 is a synthetic triacylated lipopeptide (LP) that mimics the acylated amino terminus of bacterial LPs.

Pam3CSK4 is a potent activator of the proinflammatory transcription factor NF-κB.

Activation is mediated by the interaction between TLR2 and TLR1 which recognize LPs with three fatty acids, a structural characteristic of bacterial LPs.

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Specifications

Specificity: TLR 1/2 agonist

Working concentration: 1 ng - 300 ng/ml

Molecular formula: C118H199N14O19S, 2 TFA

Molecular weight: 2150

Endotoxin level:  <0.001 EU/μg 

Spectral properties of carboxy tetramethyl rhodamine

• Excitation λ max: 549 nm
• Emission λ max: 566 nm

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Contents

•  50 μg Pam3CSK4 Rhodamine x 2 TFA
• 1.5 ml endotoxin-free water

Pam3CSK4 Rhodamine is provided lyophilized and shipped at room temperature.

Store at 4°C.

Protect from light.

Upon resuspension, prepare aliquots of Pam3CSK4 Rhodamine and store at 4°C for short term storage or -20°C for long storage.

Resuspended product is stable 1 month at 4°C and 6 months at -20°C when properly stored.

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Description

Bacterial lipoproteins are a family of proinflammatory cell wall components found in both Gram positive and Gram negative bacteria.

The stimulatory activity of bacterial lipoproteins resides in their acylated amino terminus. Pam3CSK4 is a synthetic tripalmitoylated lipopeptide that mimicks the acylated amino terminus of bacterial lipoproteins.

Pam3CysSerLys4 (Pam3CSK4) is a potent activator of the proinflammatory transcription factor NF-κB [1].

Recognition of Pam3CSK4 is mediated by TLR2 which cooperates with TLR1 through their cytoplasmic domain to induce the signaling cascade leading to the activation of NF-κB [2].

 

1. Aliprantis AO et al., 1999. Cell activation and apoptosis by bacterial lipoproteins through toll-like receptor-2. Science.285(5428):736-9.
2. Ozinsky A. et al., 2000. The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between toll-like receptors. PNAS. 97(25):13766-71.

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