PAb-hTLR6

This antibody has been removed from our catalog. However, you may contact us if you wish to order minimal quantities.

Human TLR6 Neutralizing antibody - polyclonal rat IgG

PAb hTLR6 is a polyclonal antibody specific for human Toll-like receptor 6 (hTLR6).

PAb hTLR6 was generated by DNA vaccination. Wistar rats received four hydrodynamic injections of pVAC-hTLR6, a plasmid expressing the extracellular region of hTLR6. The sera were harvested and the IgG fraction purified by Protein G affinity chromatography.

Specifications

Application: Neutralization of human TLR6-induced cellular activation

Specificity: Human TLR6

Isotype: Rat IgG

Formulation: H₂O with 250 U/ml Pen and 250 μg/ml Strep

Contents

• 200 μg polyclonal anti-hTLR6 antibody (PAb-hTLR6), provided sterile, azide-free and lyophilized.

Product is shipped at room temperature.

Lyophilized PAb-hTLR6 should be stored at -20°C.

Product is stable for 6 months. Resuspended PAb-hTLR6 should be stored at 4°C for 1 month or at -20°C for 3 months.

Description

Toll-like receptor (TLR) 6, which is closely related to TLR1, is expressed predominantly in spleen, thymus, ovary and lung [1]. TLR6 cooperates with TLR2 to recognize diacylated lipoproteins (LPs) found in mycoplasma but is not required in the recognition of triacylated LPs found in bacteria [2]. This suggests that TLR6 is able to discriminate between the N- terminal lipoylated structures of LPs derived from mycoplasma and bacteria. TLR6 collaboration with TLR2 is essential for the recognition and signal transduction of diacylated LPs, such as MALP- 2 and FSL-1 [2, 3], as well as heat-killed mycoplasma, indicating that TLR6 and TLR2 play a central role in recognizing mycoplasma [2].

1. Takeuchi O. et al., 1999. TLR6: A novel member of an expanding toll-like receptor family. Gene. 231(1-2):59-65.
2. Takeuchi O. et al., 2001. Discrimination of bacterial lipoproteins by Toll-like receptor 6. Int Immunol, 13(7):933-40.
3. Fujita M. et al., 2003. Involvement of leucine residues at positions 107, 112, and 115 in a leucine-rich repeat motif of human Toll-like receptor 2 in the recognition of diacylated lipoproteins and lipopeptides and Staphylococcus aureus peptidoglycans. J Immunol. 171(7):3675-83.