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PAb-hTLR5

PAb-hTLR5 Unit size Cat. code Docs Qty Price
Human TLR5 Neutralizing antibody - Polyclonal Rat IgG
200 µg
pab-hstlr5
+-
$244.00

Human TLR5 Neutralizing antibody - Polyclonal Rat IgG

PAb hTLR5 is a polyclonal antibody specific for human Toll-like receptor 5 (TLR5).

PAb hTLR5 was generated by DNA vaccination. Wistar rats received four hydrodynamic injections of pVAC-hTLR5, a plasmid expressing the extracellular region of human TLR5.

The sera were harvested and the IgG fraction purified by Protein G affinity chromatography.

The control for use with PAb-hTLR5 is PAb Control.

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Specifications

Clonality: Polyclonal antibody

Applications: Neutralization of human TLR5-induced cellular activation

Specificity: Human TLR5

Isotype: Rat IgG

Formulation: H2O with 250 U/ml Pen and 250 μg/ml Strep

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Contents

  • 200 μg polyclonal anti-hTLR5 antibody (PAb-hTLR5), provided sterile, azide-free and lyophilized.

room temperature Product is shipped at room temperature.

store Lyophilized PAb-hTLR5 should be stored at -20°C. Resuspended PAb-hTLR5 should be stored at -20°C for 1 year.

stability Product is stable for 1 year.

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Description

Toll-like receptors (TLRs) play a critical role in early innate immunity to invading pathogens by sensing microorganisms. These evolutionary conserved receptors recognize highly conserved structural motifs only expressed by microbial pathogens, called pathogen-associated microbial patterns (PAMPs). Stimulation of TLRs by PAMPs initiates a signaling cascade leading to the secretion of proinflammatory cytokines following NF-κB activation. To date ten human and twelve murine TLRs have been characterized, TLR1 to TLR10 in humans, and TLR1 to TLR9, TLR11, TLR12 and TLR13 in mice, the homolog of TLR10 being a pseudogene.

TLR5 recognizes flagellin from both Gram-positive and Gram-negative bacteria. Activation of the receptor stimulates the production of proinflammatory cytokines, such as TNF-α, through signaling via the adaptor protein MyD88 and the serine kinase IRAK [1, 2]. TLR5 can generate a proinflammatory signal as a homodimer suggesting that it might be the only TLR participating in flagellin recognition [2]. However, TLR5 may require the presence of a co-receptor or adaptor molecule for efficient ligand recognition and/or signaling [3].

 

1. Gewirtz AT. et al., 2001. Cutting edge: bacterial flagellin activates basolaterally expressed TLR5 to induce epithelial proinflammatory gene expression. J Immunol, 167(4):1882-5.
2. Hayashi F. et al., 2001. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature, 410(6832):1099-103.
3. Tallant T. et al., 2004. Flagellin acting via TLR5 is the major activator of key signaling pathways leading to NF-kappa B and proinflammatory gene program activation in intestinal epithelial cells. BMC Microbiol. 4(1):33.

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Citations

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