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PAb-hTLR4

PAb-hTLR4 Unit size Cat. code Docs Qty Price
Human TLR4 Neutralizing antibody - Polyclonal Rat IgG
200 µg
pab-hstlr4
+-
$253.00

Human TLR4 Neutralizing antibody - Polyclonal Rat IgG

PAb hTLR4 is a polyclonal antibody specific for human Toll-like receptor 4 (TLR4, CD284).

PAb hTLR4 was generated by DNA vaccination. Wistar rats received four hydrodynamic injections of pVAC-hTLR4, a plasmid expressing the extracellular region of human TLR4.

The sera were harvested and the IgG fraction purified by Protein G affinity chromatography.

The control for use with PAb hTLR4 is PAb Control.

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Specifications

Clonality: Polyclonal antibody

Applications: Neutralization of human TLR4-induced cellular activation

Specificity: Human TLR4

Isotype: Rat IgG

Formulation:  H2O with 250 U/ml Pen and 250 μg/ml Strep

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Contents

  • 200 μg polyclonal anti-hTLR4 antibody (PAb-hTLR4), provided sterile, azide-free and lyophilized.

room temperature Product is shipped at room temperature.

store Lyophilized PAb-hTLR4 should be stored at -20°C. Resuspended PAb-hTLR4 should be stored at -20°C for 1 year.

stability Product is stable for 1 year.

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Description

Toll-Like receptors (TLRs) play a critical role in early innate immunity to invading pathogens by sensing microorganisms. These evolutionary conserved receptors recognize highly conserved structural motifs only expressed by microbial pathogens, called pathogen-associated microbial patterns (PAMPs). Stimulation of TLRs by PAMPs initiates a signaling cascade leading to the secretion of proinflammatory cytokines following NF-κB activation. To date ten human and twelve murine TLRs have been characterized, TLR1 to TLR10 in humans, and TLR1 to TLR9, TLR11, TLR12 and TLR13 in mice, the homolog of TLR10 being a pseudogene.

TLR4, the first human TLR identified, is the receptor for Gram-negative lipopolysaccharide (LPS). The TLR4 gene was shown to be mutated in C3H/HeJ and C57BL/10ScCr mice, both of which are low responders to LPS [1]. However, TLR4 alone is not sufficient to confer LPS responsiveness. TLR4 requires MD-2, a secreted molecule, to functionally interact with LPS [2]. Furthermore, a third protein, called CD14, was shown to participate in LPS signaling, leading to NF-κB translocation. This signaling is mediated through several adaptor proteins: MyD88 TIRAP/Mal [3] , TRIF/TICAM1 and TRAM/TICAM2 [4].

 

1. Poltorak A. et al., 1998. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science, 282(5396):2085-8.
2. Shimazu R. et al., 1999. MD-2, a molecule that confers lipopolysaccharide responsiveness on Toll-like receptor 4. J Exp Med, 189(11):1777-82.
3. Horng T. GM. Barton, and R. Medzhitov, 2001. TIRAP: an adapter molecule in the Toll signaling pathway. Nat Immunol, 2(9):835-41.
4. Fitzgerald KA. et al., 2003. LPS-TLR4 Signaling to IRF-3/7 and NF-{kappa}B Involves the Toll Adapters TRAM and TRIF. J Exp Med. 198(7):1043-1055.

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