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PAb-hTLR2 [ DISCONTINUED ]

PAb-hTLR2 Unit size Cat. code Docs Qty Price
Human TLR2 Neutralizing antibody - Polyclonal Rat IgG
200 µg
pab-hstlr2
+-
$261.00
Discontinued

This product has been discontinued.
InvivoGen recommends replacing it with the monoclonal antibody Anti-hTLR2-IgA

 

 

Human TLR2 Neutralizing antibody - Polyclonal Rat IgG

PAb hTLR2 is a polyclonal antibody specific for human Toll-like receptor 2 (TLR2, CD282).

PAb hTLR2 was generated by DNA vaccination. Wistar rats received four hydrodynamic injections of pVAC-hTLR2, a plasmid expressing the extracellular region of human TLR2. The sera were harvested and the IgG fraction purified by Protein G affinity chromatography.

The control for use with PAb-hTLR2 is PAb Control.

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Specifications

Clonality: Polyclonal antibody

Applications: Neutralizing human TLR2-induced cellular activation

Specificity: Human TLR2

Isotype: Rat IgG

Formulation: H2O with 250 U/ml Pen and 250 μg/ml Strep

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Contents

  • 200 μg polyclonal anti-hTLR2 antibody (PAb-hTLR2), provided sterile, azide-free and lyophilized.

room temperature Product is shipped at room temperature.

store Lyophilized PAb-hTLR2 should be stored at -20°C.

stability Product is stable for 1 year. Resuspended PAb-hTLR2 should be stored at -20°C for 1 year.

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Description

Toll-Like receptors (TLRs) play a critical role in early innate immunity toinvading pathogens by sensing microorganisms. These evolutionary conserved receptors recognize highly conserved structural motifs only expressed by microbial pathogens, called pathogen-associated microbial patterns (PAMPs). Stimulation of TLRs by PAMPs initiates a signaling cascade leading to the secretion of proinflammatory cytokines following NF-κB activation. To date ten human and twelve murine TLRs have been characterized, TLR1 to TLR10 in humans, and TLR1 to TLR9, TLR11, TLR12 and TLR13 in mice, the homolog of TLR10 being a pseudogene.

TLR2 is involved in the recognition of a wide array of microbial molecules. TLR2 recognizes lipoteichoic acid and lipoprotein from gram- positive bacteria, lipoarabinomannan from mycobacteria, and zymosan from yeast cell wall. Moreover, TLR2 participates in the recognition of some types of LPS. TLR2 is known to heterodimerize with other TLRs, a property believed to extend the range of microbial molecules that TLR2 can recognize. TLR2 cooperates with TLR6 in response to diacylated mycoplasmal lipopeptide [1], and associates with TLR1 to recognize triacylated lipopetides [2]. Furthermore, pathogen recognition by TLR2 is strongly enhanced by CD14 [3].

 

1. Girard R et al., 2003. Lipopolysaccharides from Legionella and Rhizobium stimulate mouse bone marrow granulocytes via Toll-like receptor 2. J Cell Sci. 116(Pt 2):293-302.
2. Ozinsky A. et al., 2000. The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between toll-like receptors. Proc Natl Acad Sci USA. 97(25):13766-71.
3. Lotz S. et al., 2004. Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2. J Leukoc Biol. 75(3):467-77.

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Citations

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