Invivogen
Menu

ORN Sa19 Control

ORN Sa19 Control Unit size Cat. code Docs Qty Price
Negative control for ORN Sa19
200 µg
tlrl-orn19c
+-
$144.00

Negative control for ORN Sa19

ORN Sa19 Control is a 19 mer S. aureus 23S rRNA derived oligoribonucleotide, which carries a G in place of the central A.

ORN Sa19 Control is stabilized by phosphorothioate modification.

ORN Sa19 Control can be used as a negative control for ORN Sa19.

Back to the top

Specifications

Working concentration: 0.02-2 μg/ml ORN Sa19 Control.

ORN Sa19 Control sequence
5’ -GGACGGGAAGACCCCGUGG- 3’

ORN Sa19 Control is stabilized by phosphorothioate modification.

Back to the top

Contents

ORN Sa19 Control is provided lyophilized:

  • 200 μg lyophilized ORN Sa19 Control.
  • 2 ml endotoxin-free water.

ORN Sa19 Control is shipped at room temperature.

Upon receipt it should be stored at -20°C.

Back to the top

Description

Toll-like receptor 13 is an endosomal murine TLR which role and ligand remain unclear.

Three independent teams have identified bacterial single-stranded RNA as a TLR13 ligand [1, 2, 3].

More precisely, a conserved 23S ribosomal RNA (rRNA) sequence, “CGGAAAGACC”, was shown to induce cytokine production in a TLR13-, MyD88 and UNC93B-dependent manner.

Interestingly, this rRNA sequence is the binding site of the macrolide-lincosamide-streptogramin (MLS) group of antibiotics, and 23S rRNA from bacteria resistant to these antibiotics is not recognized by TLR13 [1].

Further, these data reveal a mechanism developed by bacteria to evade host innate immune system through acquisition of antibiotic resistance.

 

1. Oldenburg M. et al., 2012. TLR13 recognizes bacterial 23S rRNA devoid of erythromycin resistance-forming modification. Science. 337(6098).
2. Hidmark A. et al., 2012. Cutting edge: TLR13 is a receptor for bacterial RNA. J Immunol. 189(6):2717-21.
3. Li XD & Chen ZJ. 2012. Sequence specific detection of bacterial 23S ribosomal RNA by TLR13. elife. 1:e00102.

Back to the top

Customer Service
& Technical Support

CAPTCHA
In order to prevent automated spam submissions, please answer the following question:
5 + 8 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.
Shopping cart is empty