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ORN Sa19

ORN Sa19 Unit size Cat. code Docs Qty Price
TLR13 Agonist - 23S rRNA derived oligoribonucleotide
200 µg
tlrl-orn19
+-
$216.00

TLR13 Agonist - 23S rRNA derived oligoribonucleotide

ORN Sa19 is a 19 mer S. aureus 23S rRNA derived oligoribonucleotide, ORN Sa19, which contains an A in its center to mirror S. aureus A2085, is highly stimulatory in TLR13-expressing cells which carries a G in place of the central A.

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Specifications

Specificity: murine TLR13 agonist

Working concentration: 0.02-2 μg/ml

ORN Sa19 sequence:

ORN Sa19 is a 19 mer S. aureus 23S rRNA derived oligoribonucleotide, stabilized by phosphorothioate modification.
Sequence 5’ -GGACGGAAAGACCCCGUGG- 3’

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Contents

ORN Sa19 is provided lyophilized :

  • 200 μg lyophilized ORN Sa19.
  • 1.5 ml endotoxin-free water.

ORN Sa19 is shipped at room temperature.

Upon receipt, store at -20°C.

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Description

Toll-like receptor 13 is an endosomal murine TLR which role and ligand remain unclear.

Three independent teams have identified bacterial single-stranded RNA as a TLR13 ligand [1, 2, 3].

More precisely, a conserved 23S ribosomal RNA (rRNA) sequence, “CGGAAAGACC”, was shown to induce cytokine production in a TLR13-, MyD88, and UNC93B-dependent manner.

Interestingly, this rRNA sequence is the binding site of the macrolide-lincosamide-streptogramin (MLS) group of antibiotics, and 23S rRNA from bacteria resistant to these antibiotics is not recognized by TLR13 [1].

Further, these data reveal a mechanism developed by bacteria to evade the host innate immune system through the acquisition of antibiotic resistance.

 

1. Oldenburg M. et al., 2012. TLR13 recognizes bacterial 23S rRNA devoid of erythromycin resistance-forming modification. Science. 337(6098).
2. Hidmark A. et al., 2012. Cutting edge: TLR13 is a receptor for bacterial RNA. J Immunol. 189(6):2717-21.
3. Li XD & Chen ZJ. 2012. Sequence specific detection of bacterial 23S ribosomal RNA by TLR13. elife. 1:e00102.

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Citations

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