ODN 2216 FITC

FITC labeled CpG oligonucleotide - Human TLR9 ligand

CpG  ODNs are synthetic oligonucleotides that contain unmethylated CpG  dinucleotides in particular sequence contexts (CpG motifs).

These CpG  motifs are present at a 20-fold greater frequency in bacterial DNA  compared to mammalian DNA. They have been shown to induce a coordinated  set of immune responses based on the activation of immune cells  primarily involved in the recognition of these molecules.

ODN  2216 FITC is a A-class CpG ODN with a preference for human TLR9.

ODN 2216 FITC can  be used to evaluate CpG ODN cellular uptake and localization by  confocal laser-scanning microscopy (excitation 495 nm, emission 520 nm)  or flow cytometry.

Specifications

Specificity: human TLR9 agonist

Working concentration: 1-5 μM

ODN 2216 sequence : 5’-ggGGGACGA:TCGTCgggggg-3’ (20 mer)
Note: Bases in capital letters are phosphodiester and those in lower case are phosphorothioate.

Spectral Properties of FITC
Excitation λ max: 495 nm
Emission λ max: 520 nm

Contents

• 50 μg (7 nmol) lyophilized ODN 2216 labeled with FITC at the 3’ end
• 1 ml sterile endotoxin-free water

ODN 2216 FITC is shipped at room temperature.

Stored at -20°C. Upon resuspension, ODN 2216 FITC should be aliquoted and stored at -20°C.

Resuspended product is stable 6 months when properly stored.

Protect from light.  Avoid repeated freeze-thaw cycles.