ODN 2216 FITC
|ODN 2216 FITC||Unit size||Cat. code||Docs||Qty||Price|
Class A - FITC-labeled ODN 2216
FITC labeled CpG oligonucleotide - Human TLR9 ligand
CpG ODNs are synthetic oligonucleotides that contain unmethylated CpG dinucleotides in particular sequence contexts (CpG motifs).
These CpG motifs are present at a 20-fold greater frequency in bacterial DNA compared to mammalian DNA. They have been shown to induce a coordinated set of immune responses based on the activation of immune cells primarily involved in the recognition of these molecules.
ODN 2216 FITC is a A-class CpG ODN with a preference for human TLR9.
ODN 2216 FITC can be used to evaluate CpG ODN cellular uptake and localization by confocal laser-scanning microscopy (excitation 495 nm, emission 520 nm) or flow cytometry.
Specificity: human TLR9 agonist
Working concentration: 1-5 μM
ODN 2216 sequence : 5’-ggGGGACGA:TCGTCgggggg-3’ (20 mer)
Note: Bases in capital letters are phosphodiester and those in lower case are phosphorothioate.
Spectral Properties of FITC
Excitation λ max: 495 nm
Emission λ max: 520 nm
- 50 μg (7 nmol) lyophilized ODN 2216 labeled with FITC at the 3’ end
- 1 ml sterile endotoxin-free water
ODN 2216 FITC is shipped at room temperature.
Stored at -20°C. Upon resuspension, ODN 2216 FITC should be aliquoted and stored at -20°C.
Resuspended product is stable 6 months when properly stored.
Protect from light. Avoid repeated freeze-thaw cycles.Back to the top